Spero is a surname. Notable people with the surname include:

• Anthony Spero (1929-2008), American criminal
• David Spero (born 1951), American DJ and music manager
• Deborah Spero, American law enforcement official
• Donald Spero (born 1939), American rower and venture capitalist
• Emji Spero, American queer performance artist
• Ernest Spero (1894-1960), British politician
• George Spero (footballer) (born 1941), Australian rules footballer
• Greg Spero (born 1985), American pianist and composer
• Joan E. Spero (born 1944), American diplomat
• Nancy Spero (1926–2009), American visual artist
• Shubert Spero (born 1923), American rabbi
• Stanislas Spero Adotevi (born 1934), Beninese politician
• Wendy Spero, American comedian

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