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词条 Thermus thermophilus
释义

  1. Biotechnological applications of T. thermophilus enzymes

  2. References

  3. External links

{{Taxobox
| regnum = Bacteria
| phylum = Deinococcus-Thermus
| classis = Deinococci
| ordo = Thermales
| familia = Thermaceae
| genus = Thermus
| species = T. thermophilus
| binomial = Thermus thermophilus
}}Thermus thermophilus is a Gram negative bacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural genomics, and systems biology. The bacterium is extremely thermophilic, with an optimal growth temperature of about {{convert|65|C}}. Thermus thermophilus was originally isolated from a thermal vent within a hot spring in Izu, Japan by Tairo Oshima and Kazutomo Imahori.[1] The organism has also been found to be important in the degradation of organic materials in the thermogenic phase of composting.[2]T. thermophilus is classified into several strains, of which HB8 and HB27 are the most commonly used in laboratory environments. Genome analyses of these strains were independently completed in 2004.[3]

Biotechnological applications of T. thermophilus enzymes

  • rTth DNA polymerase is a recombinant thermostable DNA polymerase derived from Thermus thermophilus, with optimal activity at 70-80 °C, used in some PCR applications. The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.

References

1. ^{{cite journal|doi=10.1099/00207713-24-1-102|journal=Int J Syst Bacteriol|date=1974|volume=24|pages=102–112|title=Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from Japanese thermal spa.|first2=K.|last2=Imahori|author=Oshima|last-author-amp=yes}}
2. ^{{cite journal|journal=Appl. Environ. Microbiol.|date=1996|volume=62|pages=1723–1727|title=Isolation of Thermus strains from hot composts (60ºC-80ºC)|display-authors=6|author=Beffa, T|author2=Blanc, M|author3=Lyon, PF|author4=Vogt, G|author5=Marchiani, M|author6=Fischer, JL|author7=Aragno, M|pmid=8633870|issue=5|pmc=167946}}
3. ^{{cite journal|journal=Nat Biotechnol|date=May 2004|volume=22|issue=5|pages=547–53|title=The genome sequence of the extreme thermophile Thermus thermophilus|author=Henne A|display-authors=etal|pmid=15064768|doi=10.1038/nbt956}}

External links

  • Thermus thermophilus HB27 genome page.
  • Thermus thermophilus HB8 genome page.
  • Structural-biological whole cell project of Thermus thermophilus HB8.
  • Isolation of Thermus strains from hot composts (60ºC-80ºC)
  • Whole Cell Project of Thermus thermophilus HB8: Database
  • Type strain of Thermus thermophilus at BacDive - the Bacterial Diversity Metadatabase
{{Extremophile}}{{Taxonbar|from=Q139470}}

3 : Model organisms|Thermophiles|Thermozoa

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