AE1 or AE-1 is a designation used to refer to multiple things:

• HMAS AE1, the first submarine to serve in the Royal Australian Navy
• {{USS|Pyro|AE-1}}, a US ammunition ship
• Aero Ae 01, a design of Czechoslovakian military trainer biplane entering use in 1919
• Canon AE-1, a 35 mm film, single-lens reflex (SLR) camera
• Anion Exchanger 1, a transport protein
• A size designation for Constantinian bronze coins

• Maryhill F.C.
• Maryhill Line
• Maryhill, WA
• Maryhill, Washington
• Mary Hitchcock Medical Center
• Mary (Holy Mary)
• Mary Honeyball
• Mary Hopkin
• Mary Howarth Arden
• Mary Humes
• Mary Hunt
• Mary Hunter Austin
• Mary hunter austin
• Mary Hunter Wolf
• Mary Hutchinson Women's Prison
• Mary I Francisca
• Mary I Francisca of Portugal
• Mary II
• Mary II da Glória
• Mary II da Glória of Portugal
• Mary II of England
• Mary II of Portugal
• Mary II of Scotland
• Mary II of the United Kingdom
• Mary II, Queen of England