词条 | Vero cell |
释义 |
CharacteristicsThe Vero cell lineage is continuous and aneuploid, meaning that it has an abnormal number of chromosomes. A continuous cell lineage can be replicated through many cycles of division and not become senescent.[4] Vero cells are interferon-deficient; unlike normal mammalian cells, they do not secrete interferon alpha or beta when infected by viruses.[5] However, they still have the Interferon-alpha/beta receptor, so they respond normally when interferon from another source is added to the culture. The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014.[6] Chromosome 12 of Vero cells has a homozygous ~9-Mb deletion, causing the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitors CDKN2A and CDKN2B in the genome.[6] Although African green monkeys were previously classified as Cercopithecus aethiops, they have been placed within the genus Chlorocebus, which includes several species.[7] The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus.[6] Usage in ResearchVero cells are used for many purposes, including:
Lineages
Isolated from C. aethiops kidney on 27 Mar 1962
Isolated from Vero in 1968, it grows to a lower saturation density (cells per unit area) than the original Vero. It is useful for detecting and counting hemorrhagic fever viruses by plaque assays.
This line is a clone from Vero 76. Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly.
Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein glycoprotein-H (gH).[8] Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV-1 glycoprotein-D (gD) promoter region. In Vero lineage F6, expression of gH is under the control of the promoter region of gD. (Also F6B2; obs. F6B1.1) See also
References1. ^History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf 2. ^{{cite journal |vauthors=Yasumura Y, Kawakita M | title = The research for the SV40 by means of tissue culture technique | journal = Nippon Rinsho | year = 1963 | volume = 21 | issue = 6 | pages = 1201–1219 | url= https://scholar.google.com/scholar?q=Yasumura+Kawakita&ie=UTF-8&oe=UTF-8&hl=en&btnG=Search }} 3. ^{{cite book|author = Shimizu B|veditors=Seno K, Koyama H, Kuroki T |title=Manual of selected cultured cell lines for bioscience and biotechnology|language=Japanese|year=1993|publisher=Kyoritsu Shuppan|location=Tokyo|pages=299–300|isbn=978-4-320-05386-1|url=https://www.amazon.co.jp/gp/switch-language/product/4320053869/?ie=UTF8&language=en%5FJP}} 4. ^{{cite web | title = Main Types of Cell Culture | work = Fundamental Techniques in Cell Culture: a Laboratory Handbook | url = http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_5.html | accessdate=2006-09-28}} 5. ^{{cite journal |vauthors=Desmyter J, Melnick JL, Rawls WE |title=Defectiveness of Interferon Production and of Rubella Virus Interference in a Line of African Green Monkey Kidney Cells (Vero) |journal=J. Virol. |volume=2 |issue=10 |pages=955–61 |date=October 1968 |pmid=4302013 |pmc=375423 |doi= }} 6. ^1 2 3 4 {{Cite journal |vauthors=Osada N, Kohara A, Yamaji T, Hirayama N, Kasai F, Sekizuka T, Kuroda M, Hanada K |title=The genome landscape of the African green monkey kidney-derived Vero cell line |journal=DNA Research |volume=21 |issue=6 |pages=673–83 |year=2014 |doi=10.1093/dnares/dsu029 |pmid=25267831 |pmc=4263300}} 7. ^{{cite journal | vauthors = Haus T, Akom E, Agwanda B, Hofreiter M, Roos C, Zinner D | title = Mitochondrial diversity and distribution of African green monkeys (chlorocebus gray, 1870) | journal = Am. J. Primatol. | volume = 75 | issue = 4 | pages = 350–60 | date = April 2013 | pmid = 23307319 | pmc = 3613741 | doi = 10.1002/ajp.22113 }} 8. ^{{cite journal | vauthors = Forrester A, Farrell H, Wilkinson G, Kaye J, Davis-Poynter N, Minson T | title = Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted | journal = J. Virol. | volume = 66 | issue = 1 | pages = 341–8 | date = January 1992 | pmid = 1309250 | pmc = 238293 }} External links{{Commons category|Vero cells}}
1 : Monkey cell lines |
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