| 词条 | Vibrio natriegens |
| 释义 |
| color = lightgrey | name = Vibrio natriegens | regnum = Bacteria | phylum = Proteobacteria | classis = Gammaproteobacteria | ordo = Vibrionales | familia = Vibrionaceae | genus = Vibrio | binomial = Vibrio natriegens | binomial_authority = [1][2][3] | synonyms = Pseudomonas natriegens [1] Beneckea natriegens [1][4] }}Vibrio natriegens is a Gram-negative marine bacterium.[3] It was first isolated from salt marsh mud. It is a salt-loving organism (halophile) requiring about 2% NaCl for growth. It reacts well to the presence of sodium ions which appear to stimulate growth in Vibrio species, to stabilise the cell membrane, and to affect sodium-dependent transport and mobility. Under optimum conditions, and all nutrients provided, the doubling time of V. natriegens can be less than 10 minutes. In the laboratory, the growth medium can be easily changed, thus affecting the growth rate of a culture.[5][6] V. natriegens is commonly found in estuarine mud. Biotechnological UsesOwing to its rapid growth rate, ability to grow on inexpensive carbon sources, and capacity to secrete proteins into the growth media, efforts are underway to leverage this species as a host for molecular biology and biotechnology applications.[7][8] Recently, V. natriegens crude extract has been shown by multiple research groups to be a promising platform for cell-free expression.[9][10][11][12] References1. ^1 2 {{cite journal|date= 1961 |vauthors=Payne WJ, Eagon RG, Williams AK|title=Some observations on the physiology of Pseudomonas natriegens nov. spec.|journal=Antonie van Leeuwenhoek|volume=27|pages=121–8 |pmid= 13733692|doi=10.1007/bf02538432}} 2. ^{{cite journal|journal= J. Bacteriol. |date=July 1981|volume=147|issue=1|pages=36–45| title=Evolution of alkaline phosphatase in marine species of Vibrio | vauthors=Woolkalis MJ, Baumann P|pmid=6787029|pmc=216004}} 3. ^1 {{cite journal| journal=Curr. Microbiol. |volume=4|issue=3| year=1980|pages=127–132|title=Reevaluation of the taxonomy of Vibrio, Beneckea, and Photobacterium: Abolition of the genus Beneckea| vauthors=Baumann P, Baumann L, Bang SS, Woolkalis MJ| doi=10.1007/bf02602814}} 4. ^{{cite journal|title=Taxonomy of marine bacteria: the genus Beneckea| vauthors=Baumann P, Baumann L, Mandel M |journal=J. Bacteriol. |date=July 1971|volume=107| pages=268–94|pmid = 4935323|issue=1|pmc=246914}} 5. ^{{cite journal|journal=J. Bacteriol. |date= Mar 2002 | pmid = 11844764| volume=184|pages=1349–58| title=rRNA promoter activity in the fast-growing bacterium Vibrio natriegens| vauthors=Aiyar SE, Gaal T, Gourse RL| issue=5| pmc=134863| doi=10.1128/jb.184.5.1349-1358.2002}} 6. ^{{cite journal|journal=J. Bacteriol. | volume= 83 |pages=736–737| year=1962|url=http://jb.asm.org/content/83/4/736.abstract|title=Pseudomonas natriegens, A Marine Bacterium with a Generation Time of Less Than 10 Minutes| author=R. G. Eagon| issue=4}} 7. ^{{Cite journal|last=Lee|first=Henry H.|last2=Ostrov|first2=Nili|last3=Wong|first3=Brandon G.|last4=Gold|first4=Michaela A.|last5=Khalil|first5=Ahmad S.|last6=Church|first6=George M.|date=2016-06-12|title=Vibrio natriegens, a new genomic powerhouse|journal=BioRxiv|doi=10.1101/058487}} 8. ^{{Cite journal|last=Weinstock|first=Matthew T.|last2=Hesek|first2=Eric D.|last3=Wilson|first3=Christopher M.|last4=Gibson|first4=Daniel G.|date=2016-08-29|title=Vibrio natriegens as a fast-growing host for molecular biology|journal=Nature Methods|doi=10.1038/nmeth.3970|issn=1548-7105|pmid=27571549|volume=13|issue=10|pages=849–51}} 9. ^{{Cite journal|last=Wiegand|first=Daniel J.|last2=Lee|first2=Henry H.|last3=Ostrov|first3=Nili|last4=Church|first4=George M.|date=2018-09-12|title=Establishing a Cell-free Vibrio natriegens Expression System|journal=ACS Synthetic Biology|volume=7|issue=10|pages=2475–2479|doi=10.1021/acssynbio.8b00222|pmid=30160938}} 10. ^{{Cite journal|last=Wiegand|first=Daniel J.|last2=Lee|first2=Henry H.|last3=Ostrov|first3=Nili|last4=Church|first4=George M.|date=2019-03-15|title=Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens|journal=Journal of Visualized Experiments|issue=145|doi=10.3791/59495|}} 11. ^{{Cite journal|last=Des Doye|first=BJ|last2=Davidson|first2=SR|last3=Weinstock|first3=MT|last4=Gibson|first4=DG|last5=Jewett|first5=MC|date=2018-09-06|title=Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens.|journal=ACS Synthetic Biology|volume=7|issue=9|pages=2245–2255|doi=10.1021/acssynbio.8b00252|pmid=30107122}} 12. ^{{Cite journal|last=Failmezger|first=J|last2=Scholz |first2=S|last3= Blombach |first3=B|last4=Siemann-Herzberg|first4=M|date=2018-06-01|title=Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens.|journal=Frontiers in Microbiology|volume=9|doi=10.3389/fmicb.2018.01146|pmid=29910785}} External links
1 : Vibrionales |
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