- References
| Name = 2'-phosphotransferase
| EC_number = 2.7.1.160
| CAS_number =
| IUBMB_EC_number = 2/7/1/160
| GO_code =
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In enzymology, a 2'-phosphotransferase ({{EC number|2.7.1.160}}) is an enzyme that catalyzes the chemical reaction
2'-phospho-[ligated tRNA] + NAD+mature tRNA + ADP-ribose 1,2-phosphate + nicotinamide + H2O
Thus, the two substrates of this enzyme are 2'-phospho-[ligated tRNA] and NAD+, whereas its 4 products are mature tRNA, ,2-phosphate">ADP-ribose 1,2-phosphate, nicotinamide, and H2O.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is 2'-phospho-[ligated tRNA]:NAD+ phosphotransferase. Other names in common use include yeast 2'-phosphotransferase, Tpt1, Tpt1p, and 2'-phospho-tRNA:NAD+ phosphotransferase.
References
- {{cite journal |vauthors=Steiger MA, Kierzek R, Turner DH, Phizicky EM | date = 2001 | title = Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog | journal = Biochemistry | volume = 40 | pages = 14098–105 | pmid = 11705403 | doi = 10.1021/bi011388t | issue = 46 }}
- {{cite journal |vauthors=Spinelli SL, Kierzek R, Turner DH, Phizicky EM | date = 1999 | title = Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast | journal = J. Biol. Chem. | volume = 274 | pages = 2637–44 | pmid = 9915792 | doi = 10.1074/jbc.274.5.2637 | issue = 5 }}
- {{cite journal |vauthors=Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM | date = 1997 | title = A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae | journal = J. Biol. Chem. | volume = 272 | pages = 13203–10 | pmid = 9148937 | doi = 10.1074/jbc.272.20.13203 | issue = 20 }}
- {{cite journal |vauthors=McCraith SM, Phizicky EM | date = 1991 | title = An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule | journal = J. Biol. Chem. | volume = 266 | pages = 11986–92 | pmid = 2050693 | issue = 18 }}
- {{cite journal |vauthors=Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY | date = 2003 | title = A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis | journal = Cell. Mol. Life Sci. | volume = 60 | pages = 1725–32 | pmid = 14504659 | doi = 10.1007/s00018-003-3107-7 | issue = 8 }}
- {{cite journal |vauthors=Steiger MA, Jackman JE, Phizicky EM | date = 2005 | title = Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism | journal = RNA | volume = 11 | pages = 99–106 | pmid = 15611300 | doi = 10.1261/rna.7194605 | issue = 1 | pmc = 1370695 }}
- {{cite journal |vauthors=Sawaya R, Schwer B, Shuman S | date = 2005 | title = Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1 | journal = RNA | volume = 11 | pages = 107–13 | pmid = 15611301 | doi = 10.1261/rna.7193705 | issue = 1 | pmc = 1370696 }}
- {{cite journal |vauthors=Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S | date = 2005 | title = Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1 | journal = J. Mol. Biol. | volume = 348 | pages = 295–305 | pmid = 15811369 | doi = 10.1016/j.jmb.2005.02.049 | issue = 2 }}
3 : EC 2.7.1|NADH-dependent enzymes|Enzymes of unknown structure