| 释义 |
- References
- External links
- Further reading
{{Infobox_gene}}1-acyl-sn-glycerol-3-phosphate acyltransferase gamma is an enzyme that in humans is encoded by the AGPAT3 gene.[1]The protein encoded by this gene is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. The encoded protein may be an integral membrane protein. Two transcript variants encoding the same protein have been found for this gene.[1] ==Model organisms== Agpat3 knockout mouse phenotype| Characteristic | Phenotype |
|---|
| Homozygote viability | Abnormal | | Recessive lethal study | Normal | | Fertility | Normal | | Body weight | Normal | | Anxiety | Normal | | Neurological assessment | Normal | | Grip strength | Normal | | Hot plate | Normal | | Dysmorphology | Normal | | Indirect calorimetry | Normal | | Glucose tolerance test | Normal | | Auditory brainstem response | Normal | | DEXA | Normal | | Radiography | Normal | | Body temperature | Normal | | Eye morphology | Normal | | Clinical chemistry | Normal | | Plasma immunoglobulins | Normal | | Haematology | Normal | | Micronucleus test | Normal | | Heart weight | Normal | | Skin Histopathology | Normal | | Brain histopathology | Normal | | Salmonella infection | Normal[2] | | Citrobacter infection | Normal[3] | | All tests and analysis from[4][5] | Model organisms have been used in the study of AGPAT3 function. A conditional knockout mouse line, called Agpat3tm1a(EUCOMM)Wtsi[6][7] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[8][9][10]Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[4][11] Twenty five tests were carried out on mutant mice and one significant abnormality was observed.[4] Few homozygous mutant animals survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no additional significant abnormalities were observed in these.[4] References1. ^1 {{cite web | title = Entrez Gene: AGPAT3 1-acylglycerol-3-phosphate O-acyltransferase 3| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=56894| accessdate = }}
2. ^{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBGV/salmonella-challenge/ |title=Salmonella infection data for Agpat3 |publisher=Wellcome Trust Sanger Institute}}
3. ^{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBGV/citrobacter-challenge/ |title=Citrobacter infection data for Agpat3 |publisher=Wellcome Trust Sanger Institute}}
4. ^1 2 3 {{cite journal | doi = 10.1111/j.1755-3768.2010.4142.x | title = The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice | year = 2010 | author = Gerdin AK | journal = Acta Ophthalmologica | volume = 88 | pages = 925–7 }}
5. ^Mouse Resources Portal, Wellcome Trust Sanger Institute.
6. ^{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Agpat3 |title=International Knockout Mouse Consortium}}
7. ^{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4432492 |title=Mouse Genome Informatics}}
8. ^{{Cite journal| last1 = Skarnes |first1 =W. C.| doi = 10.1038/nature10163 | last2 = Rosen | first2 = B.| last3 = West | first3 = A. P.| last4 = Koutsourakis | first4 = M.| last5 = Bushell | first5 = W.| last6 = Iyer | first6 = V.| last7 = Mujica | first7 = A. O.| last8 = Thomas | first8 = M.| last9 = Harrow | first9 = J.| last10 = Cox | first10 = T.| last11 = Jackson | first11 = D.| last12 = Severin | first12 = J.| last13 = Biggs | first13 = P.| last14 = Fu | first14 = J.| last15 = Nefedov | first15 = M.| last16 = De Jong | first16 = P. J.| last17 = Stewart | first17 = A. F.| last18 = Bradley | first18 = A. | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–342 | year = 2011 | pmid = 21677750 | pmc =3572410 }}
9. ^{{cite journal | doi = 10.1038/474262a | title = Mouse library set to be knockout | year = 2011 | author = Dolgin E | journal = Nature | volume = 474 | issue = 7351 | pages = 262–3 | pmid = 21677718 }}
10. ^{{cite journal | doi = 10.1016/j.cell.2006.12.018 | title = A Mouse for All Reasons | year = 2007 | journal = Cell | volume = 128 | pages = 9–13 | pmid = 17218247 |vauthors=Collins FS, Rossant J, Wurst W | issue = 1 }}
11. ^{{cite journal|vauthors=van der Weyden L, White JK, Adams DJ, Logan DW | title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | pmc=3218837}}
External links- {{UCSC gene info|AGPAT3}}
Further reading{{refbegin | 2}}- {{cite journal | author=Leung DW |title=The structure and functions of human lysophosphatidic acid acyltransferases |journal=Front. Biosci. |volume=6 |issue= |pages= D944–53 |year= 2001 |pmid= 11487472 |doi=10.2741/Leung }}
- {{cite journal |vauthors=Wistow G, Bernstein SL, Ray S |title=Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epithelium |journal=Mol. Vis. |volume=8 |issue= |pages= 185–95 |year= 2002 |pmid= 12107412 |doi= |display-authors=etal}}
- {{cite journal |vauthors=Strausberg RL, Feingold EA, Grouse LH |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 |display-authors=etal}}
- {{cite journal |vauthors=Clark HF, Gurney AL, Abaya E |title=The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment |journal=Genome Res. |volume=13 |issue= 10 |pages= 2265–70 |year= 2003 |pmid= 12975309 |doi= 10.1101/gr.1293003 | pmc=403697 |display-authors=etal}}
- {{cite journal |vauthors=Ota T, Suzuki Y, Nishikawa T |title=Complete sequencing and characterization of 21,243 full-length human cDNAs |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 |display-authors=etal}}
- {{cite journal |vauthors=Lu B, Jiang YJ, Zhou Y |title=Cloning and characterization of murine 1-acyl-sn-glycerol 3-phosphate acyltransferases and their regulation by PPARα in murine heart |journal=Biochem. J. |volume=385 |issue= Pt 2 |pages= 469–77 |year= 2005 |pmid= 15367102 |doi= 10.1042/BJ20041348 | pmc=1134718 |display-authors=etal}}
- {{cite journal |vauthors=Gerhard DS, Wagner L, Feingold EA |title=The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 | pmc=528928 |display-authors=etal}}
- {{cite journal |vauthors=Agarwal AK, Barnes RI, Garg A |title=Functional characterization of human 1-acylglycerol-3-phosphate acyltransferase isoform 8: cloning, tissue distribution, gene structure, and enzymatic activity |journal=Arch. Biochem. Biophys. |volume=449 |issue= 1–2 |pages= 64–76 |year= 2006 |pmid= 16620771 |doi= 10.1016/j.abb.2006.03.014 }}
- {{cite journal |vauthors=Hu YH, Warnatz HJ, Vanhecke D |title=Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins |journal=BMC Genomics |volume=7|pages= 155 |year= 2006 |pmid= 16780588 |doi= 10.1186/1471-2164-7-155 | pmc=1526728 |display-authors=etal}}
{{refend}}{{gene-21-stub}} 1 : Genes mutated in mice |