“Glucagon”的意思、由来-开放百科全书

The pancreas releases glucagon when the concentration of insulin (and indirectly glucose) in the bloodstream falls too low. Glucagon causes the liver to convert stored glycogen into glucose, which is released into the bloodstream.^[3] High blood-glucose levels, on the other hand, stimulate the release of insulin. Insulin allows glucose to be taken up and used by insulin-dependent tissues. Thus, glucagon and insulin are part of a feedback system that keeps blood glucose levels stable. Glucagon increases energy expenditure and is elevated under conditions of stress.^[4] Glucagon belongs to the secretin family of hormones.

Function

Glucagon generally elevates the concentration of glucose in the blood by promoting gluconeogenesis and glycogenolysis.^[5] Glucagon also decreases fatty acid synthesis in adipose tissue and the liver, as well as promoting lipolysis in these tissues, which causes them to release fatty acids into circulation where they can be catabolised to generate energy in tissues such as skeletal muscle when required.^[6]

Glucose is stored in the liver in the form of the polysaccharide glycogen, which is a glucan (a polymer made up of glucose molecules). Liver cells (hepatocytes) have glucagon receptors. When glucagon binds to the glucagon receptors, the liver cells convert the glycogen into individual glucose molecules and release them into the bloodstream, in a process known as glycogenolysis. As these stores become depleted, glucagon then encourages the liver and kidney to synthesize additional glucose by gluconeogenesis. Glucagon turns off glycolysis in the liver, causing glycolytic intermediates to be shuttled to gluconeogenesis.

Glucagon also regulates the rate of glucose production through lipolysis. Glucagon induces lipolysis in humans under conditions of insulin suppression (such as diabetes mellitus type 1).^[7]

Glucagon production appears to be dependent on the central nervous system through pathways yet to be defined. In invertebrate animals, eyestalk removal has been reported to affect glucagon production. Excising the eyestalk in young crayfish produces glucagon-induced hyperglycemia.^[8]

Mechanism of action

Glucagon binds to the glucagon receptor, a G protein-coupled receptor, located in the plasma membrane. The conformation change in the receptor activates G proteins, a heterotrimeric protein with α, β, and γ subunits. When the G protein interacts with the receptor, it undergoes a conformational change that results in the replacement of the GDP molecule that was bound to the α subunit with a GTP molecule. This substitution results in the releasing of the α subunit from the β and γ subunits. The alpha subunit specifically activates the next enzyme in the cascade, adenylate cyclase.

Adenylate cyclase manufactures cyclic adenosine monophosphate (cyclic AMP or cAMP), which activates protein kinase A (cAMP-dependent protein kinase). This enzyme, in turn, activates phosphorylase kinase, which then phosphorylates glycogen phosphorylase b (PYG b), converting it into the active form called phosphorylase a (PYG a). Phosphorylase a is the enzyme responsible for the release of glucose-1-phosphate from glycogen polymers.

Additionally, the coordinated control of glycolysis and gluconeogenesis in the liver is adjusted by the phosphorylation state of the enzymes that catalyze the formation of a potent activator of glycolysis called fructose-2,6-bisphosphate.^[9] The enzyme protein kinase A (PKA) that was stimulated by the cascade initiated by glucagon will also phosphorylate a single serine residue of the bifunctional polypeptide chain containing both the enzymes fructose-2,6-bisphosphatase and phosphofructokinase-2. This covalent phosphorylation initiated by glucagon activates the former and inhibits the latter. This regulates the reaction catalyzing fructose-2,6-bisphosphate (a potent activator of phosphofructokinase-1, the enzyme that is the primary regulatory step of glycolysis)^[10] by slowing the rate of its formation, thereby inhibiting the flux of the glycolysis pathway and allowing gluconeogenesis to predominate. This process is reversible in the absence of glucagon (and thus, the presence of insulin).

Glucagon stimulation of PKA also inactivates the glycolytic enzyme pyruvate kinase in hepatocytes.^[11]

Physiology

Production

The hormone is synthesized and secreted from alpha cells (α-cells) of the islets of Langerhans, which are located in the endocrine portion of the pancreas. Production, which is otherwise freerunning, is suppressed/regulated by insulin from the adjacent beta cells. (Actually, GABA is the interlock signal chemical that prevents simultaneous insulin and glucagon production in the pancreas. It is produced by the pancreatic ß cells at the same time insulin is being produced. It prevents the α cells from being switched on).{{Citation needed|date=February 2019}}

When blood sugar drops, insulin production drops and more glucagon is produced.^[12] In rodents, the alpha cells are located in the outer rim of the islet. Human islet structure is much less segregated, and alpha cells are distributed throughout the islet in close proximity to beta cells. Glucagon is also produced by alpha cells in the stomach.^[12]

Recent research has demonstrated that glucagon production may also take place outside the pancreas, with the gut being the most likely site of extrapancreatic glucagon synthesis.^[13]

Regulation

Structure

Glucagon is a 29-amino acid polypeptide. Its primary structure in humans is: NH₂-His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-COOH.

The polypeptide has a molecular weight of 3485 daltons.^[20] Glucagon is a peptide (nonsteroid) hormone.

Glucagon is generated from the cleavage of proglucagon by proprotein convertase 2 in pancreatic islet α cells. In intestinal L cells, proglucagon is cleaved to the alternate products glicentin, GLP-1 (an incretin), IP-2, and GLP-2 (promotes intestinal growth).^[21]

Pathology

Abnormally elevated levels of glucagon may be caused by pancreatic tumors, such as glucagonoma, symptoms of which include necrolytic migratory erythema,^[22] reduced amino acids, and hyperglycemia. It may occur alone or in the context of multiple endocrine neoplasia type 1^[23]

Elevated glucagon is the main contributor to hyperglycemic ketoacidosis in undiagnosed or poorly treated type 1 diabetes. As the beta cells cease to function, insulin and pancreatic GABA are no longer present to suppress the freerunning output of glucagon. As a result, glucagon is released from the alpha cells at a maximum, causing rapid breakdown of glycogen to glucose and fast ketogenesis.^[24] It was found that a subset of adults with type 1 diabetes took 4 times longer on average to approach ketoacidosis when given somatostatin (inhibits glucagon production) with no insulin.{{cn|date=August 2018}} Inhibiting glucagon has been a popular idea of diabetes treatment, however some have warned that doing so will give rise to brittle diabetes in patients with adequately stable blood glucose.{{cn|date=August 2018}}

The absence of alpha cells (and hence glucagon) is thought to be one of the main influences in the extreme volatility of blood glucose in the setting of a total pancreatectomy.

History

In the 1920s, Kimball and Murlin studied pancreatic extracts, and found an additional substance with hyperglycemic properties. They described glucagon in 1923.^[25] The amino acid sequence of glucagon was described in the late 1950s.^[26] A more complete understanding of its role in physiology and disease was not established until the 1970s, when a specific radioimmunoassay was developed.{{citation needed|date=February 2018}}

Etymology

Kimball and Murlin coined the term glucagon in 1923 when they initially named the substance the glucose agonist.^[27]

See also

References

1. ^{{cite book|last1=Voet D, Voet JG.|title=Biochemistry|date=2011|publisher=Wiley|location=New York|edition=4th}}
2. ^{{cite book | vauthors = Reece J, Campbell N | title = Biology | edition = | publisher = Benjamin Cummings | location = San Francisco | year = 2002 | origyear = | pages = | quote = | isbn = 978-0-8053-6624-2 }}
3. ^{{cite book | last1 = Orsay | first1 = Jonathan | name-list-format = vanc | title = Biology 1: Molecules | date = 2014 | publisher = Examkrackers Inc. | isbn = 978-1-893858-70-1 | page=77 }}
4. ^{{cite journal | vauthors = Jones BJ, Tan T, Bloom SR | title = Minireview: Glucagon in stress and energy homeostasis | journal = Endocrinology | volume = 153 | issue = 3 | pages = 1049–54 | date = March 2012 | pmid = 22294753 | pmc = 3281544 | doi = 10.1210/en.2011-1979 }}
5. ^{{cite book|last1=Voet D, Voet JG|title=Biochemistry.|date=2011|publisher=Wiley|location=New York|edition=4th}}
6. ^{{cite journal|last1=HABEGGER, K. M., HEPPNER, K. M., GEARY, N., BARTNESS, T. J., DIMARCHI, R. & TSCHÖP, M. H.|title=The metabolic actions of glucagon revisited.|journal=Nature Reviews. Endocrinology|date=2010|volume=6|issue=12|pages=689–697|doi=10.1038/nrendo.2010.187|pmc=3563428}}
7. ^{{cite journal | vauthors = Liljenquist JE, Bomboy JD, Lewis SB, Sinclair-Smith BC, Felts PW, Lacy WW, Crofford OB, Liddle GW | title = Effects of glucagon on lipolysis and ketogenesis in normal and diabetic men | journal = The Journal of Clinical Investigation | volume = 53 | issue = 1 | pages = 190–7 | date = January 1974 | pmid = 4808635 | pmc = 301453 | doi = 10.1172/JCI107537 }}
8. ^{{cite journal | vauthors = Leinen RL, Giannini AJ | title = Effect of eyestalk removal on glucagon induced hyperglycemia in crayfish | journal = Society for Neuroscience Abstracts | year = 1983 | volume = 9 | pages = 604 }}
9. ^{{cite journal | vauthors = Hue L, Rider MH | title = Role of fructose 2,6-bisphosphate in the control of glycolysis in mammalian tissues | journal = The Biochemical Journal | volume = 245 | issue = 2 | pages = 313–24 | date = July 1987 | pmid = 2822019 | pmc = 1148124 | doi = 10.1042/bj2450313}}
10. ^{{cite journal | vauthors = Claus TH, El-Maghrabi MR, Regen DM, Stewart HB, McGrane M, Kountz PD, Nyfeler F, Pilkis J, Pilkis SJ | title = The role of fructose 2,6-bisphosphate in the regulation of carbohydrate metabolism | journal = Current Topics in Cellular Regulation | volume = 23 | issue = | pages = 57–86 | year = 1984 | pmid = 6327193 | doi = 10.1016/b978-0-12-152823-2.50006-4 | isbn = 9780121528232 }}
11. ^{{cite journal | vauthors = Feliú JE, Hue L, Hers HG | title = Hormonal control of pyruvate kinase activity and of gluconeogenesis in isolated hepatocytes | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 73 | issue = 8 | pages = 2762–6 | date = August 1976 | pmid = 183209 | pmc = 430732 | doi = 10.1073/pnas.73.8.2762 }}
12. ^¹{{cite journal | vauthors = Unger RH, Cherrington AD | title = Glucagonocentric restructuring of diabetes: a pathophysiologic and therapeutic makeover | journal = The Journal of Clinical Investigation | volume = 122 | issue = 1 | pages = 4–12 | date = January 2012 | pmid = 22214853 | pmc = 3248306 | doi = 10.1172/JCI60016 }}
13. ^{{cite journal | vauthors = Holst JJ, Holland W, Gromada J, Lee Y, Unger RH, Yan H, Sloop KW, Kieffer TJ, Damond N, Herrera PL | title = Insulin and Glucagon: Partners for Life | journal = Endocrinology | volume = 158 | issue = 4 | pages = 696–701 | date = April 2017 | pmid = 28323959 | doi = 10.1210/en.2016-1748 | url = https://academic.oup.com/endo/article/2965091 }}
14. ^{{cite journal | vauthors = Layden BT, Durai V, Lowe WL | title = G-Protein-Coupled Receptors, Pancreatic Islets, and Diabetes | journal = Nature Education | volume = 3 | issue = 9 | pages = 13 | year = 2010 | url = http://www.nature.com/scitable/topicpage/g-protein-coupled-receptors-pancreatic-islets-and-14257267 }}
15. ^{{cite journal | vauthors = Skoglund G, Lundquist I, Ahrén B | title = Alpha 1- and alpha 2-adrenoceptor activation increases plasma glucagon levels in the mouse | journal = European Journal of Pharmacology | volume = 143 | issue = 1 | pages = 83–8 | date = November 1987 | pmid = 2891547 | doi = 10.1016/0014-2999(87)90737-0 }}
16. ^{{cite journal | vauthors = Honey RN, Weir GC | title = Acetylcholine stimulates insulin, glucagon, and somatostatin release in the perfused chicken pancreas | journal = Endocrinology | volume = 107 | issue = 4 | pages = 1065–8 | date = October 1980 | pmid = 6105951 | doi = 10.1210/endo-107-4-1065 }}
17. ^{{cite journal | vauthors = Xu E, Kumar M, Zhang Y, Ju W, Obata T, Zhang N, Liu S, Wendt A, Deng S, Ebina Y, Wheeler MB, Braun M, Wang Q | title = Intra-islet insulin suppresses glucagon release via GABA-GABAA receptor system | journal = Cell Metabolism | volume = 3 | issue = 1 | pages = 47–58 | date = January 2006 | pmid = 16399504 | doi = 10.1016/j.cmet.2005.11.015 }}
18. ^{{cite journal | vauthors = Krätzner R, Fröhlich F, Lepler K, Schröder M, Röher K, Dickel C, Tzvetkov MV, Quentin T, Oetjen E, Knepel W | title = A peroxisome proliferator-activated receptor gamma-retinoid X receptor heterodimer physically interacts with the transcriptional activator PAX6 to inhibit glucagon gene transcription | journal = Molecular Pharmacology | volume = 73 | issue = 2 | pages = 509–17 | date = February 2008 | pmid = 17962386 | doi = 10.1124/mol.107.035568 }}
19. ^{{cite book | first = Leonard R. | last = Johnson | name-list-format = vanc | title = Essential Medical Physiology | year = 2003 | publisher = Academic Press | isbn = 978-0-12-387584-6 | pages = 643– }}
20. ^{{cite journal | vauthors = Unger RH, Orci L | title = Glucagon and the A cell: physiology and pathophysiology (first two parts) | journal = The New England Journal of Medicine | volume = 304 | issue = 25 | pages = 1518–24 | date = June 1981 | pmid = 7015132 | doi = 10.1056/NEJM198106183042504 }}
21. ^{{cite journal | vauthors = Orskov C, Holst JJ, Poulsen SS, Kirkegaard P | title = Pancreatic and intestinal processing of proglucagon in man | journal = Diabetologia | volume = 30 | issue = 11 | pages = 874–81 | date = November 1987 | pmid = 3446554 | doi = 10.1007/BF00274797 | doi-broken-date = 2019-03-15 }}
22. ^{{cite journal | vauthors = John AM, Schwartz RA | title = Glucagonoma syndrome: a review and update on treatment | journal = Journal of the European Academy of Dermatology and Venereology | volume = 30 | issue = 12 | pages = 2016–2022 | date = December 2016 | pmid = 27422767 | doi = 10.1111/jdv.13752 }}
23. ^{{cite journal | vauthors = Oberg K | title = Pancreatic endocrine tumors | journal = Seminars in Oncology | volume = 37 | issue = 6 | pages = 594–618 | date = December 2010 | pmid = 21167379 | doi = 10.1053/j.seminoncol.2010.10.014 }}
24. ^{{cite journal | vauthors = Fasanmade OA, Odeniyi IA, Ogbera AO | title = Diabetic ketoacidosis: diagnosis and management | journal = African Journal of Medicine and Medical Sciences | volume = 37 | issue = 2 | pages = 99–105 | date = June 2008 | pmid = 18939392 | doi = | url = }}
25. ^{{cite journal | vauthors = Kimball C, Murlin J | title = Aqueous extracts of pancreas III. Some precipitation reactions of insulin | journal = J. Biol. Chem. | year = 1923 | volume = 58 | pages = 337–348 | url = http://www.jbc.org/cgi/reprint/58/1/337 | issue=1}}
26. ^{{cite journal | vauthors = Bromer W, Winn L, Behrens O | title = The amino acid sequence of glucagon V. Location of amide groups, acid degradation studies and summary of sequential evidence | journal = J. Am. Chem. Soc. | year = 1957 | volume = 79 | issue = 11 | pages = 2807–2810|doi=10.1021/ja01568a038 }}
27. ^{{Cite web|url=https://www.diapedia.org/metabolism-insulin-and-other-hormones/5105141812/history-of-glucagon|title=History of glucagon - Metabolism, insulin and other hormones - Diapedia, The Living Textbook of Diabetes|website=www.diapedia.org|language=en|access-date=2017-03-26}}