| 词条 | Avian HBV RNA encapsidation signal epsilon |
| 释义 |
| Name = AHBV_epsilon cis-regulatory RNA element | image = AHBV_epsilon.jpg | width = | caption = Predicted secondary structure and sequence conservation of AHBV_epsilon | Symbol = AHBV_epsilon | AltSymbols = | Rfam = RF01313 | miRBase = | miRBase_family = | RNA_type = Cis-reg | Tax_domain = Viruses | GO = {{GO|0019079}} | SO = {{SO|0005836}} | CAS_number = | EntrezGene = | HGNCid = | OMIM = | PDB = | RefSeq = | Chromosome = | Arm = | Band = | LocusSupplementaryData = }} The Avian HBV RNA encapsidation signal epsilon (AHBV_epsilon) is an RNA structure that is shown to facilitate encapsidation of the pregenomic RNA required for replication. There are two main classes of encapsidation signals in avian HBV viruses - Duck hepatitis B virus (DHBV) and Heron hepatitis B virus (HHBV) like. DHBV is used as a model to understand human HBV. Although studies have shown that the HHBV epsilon has less pairing in the upper stem than DHBV, this pairing is not absolutely required for DHBV infection in ducks.[1] DHBV epsilon consists of a stem structure, a bulge and an apical loop (Fig. 1). The RNA structure was determined by chemical probing, NMR analysis and by mutagenesis.[2] HHBV epsilon also consists of a stem structure, a bulge and an apical loop (Fig. 2). The RNA structure has been determined by chemical probing and by mutagenesis. However, it has less base pairing in the upper stem than DHBV epsilon. [3] Recent NMR analysis of both the HHBV and DHBV epsilon have shown that the apical loop to be capped by a stable well-structured UGUU tetraloop.[4] In the Rfam database a single covariation model (AHBV_epsilon box right) best discriminates between the true instances of the element in sequence databases and background. The secondary structure diagram is a computer generated view of the structure with highly conserved bases shown in red. It includes the major elements shown in Figures 1 and 2. See also
References1. ^{{cite journal|last=Schmid|first=B|author2=Rösler, C|author3=Nassal, M|title=A high level of mutation tolerance in the multifunctional sequence encoding the RNA encapsidation signal of an avian hepatitis B virus and slow evolution rate revealed by in vivo infection.|journal=Journal of Virology|year=2011|volume=85|issue=18|pages=9300–13|pmid=21752921|doi=10.1128/JVI.05005-11|pmc=3165760}} 2. ^{{cite journal| doi= 10.1093/nar/26.21.4818| last1= Smith Gj| first1= 3rd| last2= Donello| first2= JE| last3= Lück| first3= R| last4= Steger| first4= G| last5= Hope| first5= TJ| title= The hepatitis B virus post-transcriptional regulatory element contains two conserved RNA stem-loops which are required for function| journal= Nucleic Acids Research| volume= 26| issue= 21| pages= 4818–27| year= 1998 | pmid = 9776740| pmc= 147918}} 3. ^{{cite journal| last1= Beck| first1= J| last2= Nassal| first2= M| title= Sequence- and structure-specific determinants in the interaction between the RNA encapsidation signal and reverse transcriptase of avian hepatitis B viruses| journal= Journal of Virology| volume= 71| issue= 7| pages= 4971–80| year= 1997 | pmid = 9188560| pmc= 191728}} 4. ^{{cite journal| last1= Girard| first1= FC| last2= Ottink| first2= OM| last3= Ampt| first3= KA| last4= Tessari| first4= M| last5= Wijmenga| first5= SS| title= Thermodynamics and NMR studies on Duck, Heron and Human HBV encapsidation signals| journal= Nucleic Acids Research| volume= 35| issue= 8| pages= 2800–11| year= 2007 | pmid = 17430968| pmc= 1885660| doi= 10.1093/nar/gkm131}} External links
2 : Cis-regulatory RNA elements|Hepadnaviridae |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。