| 释义 |
- Use of HNK-1 Antibody for Neural Crest Research
- References
- External links
- Further reading
{{Infobox_gene}}Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 is an enzyme that in humans is encoded by the B3GAT2 gene.[1][2]The product of this gene is a transmembrane protein belonging to the glucuronyltransferase family, and catalyzes the transfer of a beta-1,3 linked glucuronic acid to a terminal galactose in different glycoproteins or glycolipids containing a Gal-beta-1-4GlcNAc or Gal-beta-1-3GlcNAc residue. The encoded protein is involved in the synthesis of the human natural killer-1 (HNK-1) carbohydrate epitope, a sulfated trisaccharide implicated in cellular migration and adhesion in the nervous system.[2] Use of HNK-1 Antibody for Neural Crest ResearchAntibodies raised against the HNK-1 epitope have played a large role in studies of the neural crest, especially in the avian embryo.[3] The first antibody raised against this epitope was NC-1,[4] which permitted much easier analyses of neural crest migration pathways. In avians, and especially in other vertebrates, the results of HNK-1 staining should be interpreted with caution as the epitope is not unique to the neural crest. References1. ^{{cite journal | vauthors = Imiya K, Ishizaki T, Seiki T, Saito F, Inazawa J, Oka S, Kawasaki T | title = cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope | journal = Gene | volume = 296 | issue = 1-2 | pages = 29–36 |date=Oct 2002 | pmid = 12383500 | pmc = | doi =10.1016/S0378-1119(02)00840-5 }}
2. ^1 {{cite web | title = Entrez Gene: B3GAT2 beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=135152| accessdate = }}
3. ^{{cite journal |author=Bronner-Fraser M |title=Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1 |journal=Dev. Biol. |volume=115 |issue=1 |pages=44–55 |date=May 1986 |pmid=3516760 |doi= 10.1016/0012-1606(86)90226-5|url=}}
4. ^{{cite journal |vauthors=Vincent M, Thiery JP |title=A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system |journal=Dev. Biol. |volume=103 |issue=2 |pages=468–81 |date=June 1984 |pmid=6202575 |doi= 10.1016/0012-1606(84)90334-8|url=}}
External links- {{UCSC gene info|B3GAT2}}
Further reading{{refbegin | 2}}- {{cite journal | vauthors=Nagase T, Kikuno R, Ohara O |title=Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins. |journal=DNA Res. |volume=8 |issue= 6 |pages= 319–27 |year= 2002 |pmid= 11853319 |doi=10.1093/dnares/8.6.319 }}
- {{cite journal | vauthors=Strausberg RL, Feingold EA, Grouse LH |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 |display-authors=etal}}
- {{cite journal | vauthors=Marcos I, Galán JJ, Borrego S, Antiñolo G |title=Cloning, characterization, and chromosome mapping of the human GlcAT-S gene. |journal=J. Hum. Genet. |volume=47 |issue= 12 |pages= 677–80 |year= 2003 |pmid= 12522689 |doi= 10.1007/s100380200103 }}
- {{cite journal | vauthors=Mungall AJ, Palmer SA, Sims SK |title=The DNA sequence and analysis of human chromosome 6. |journal=Nature |volume=425 |issue= 6960 |pages= 805–11 |year= 2003 |pmid= 14574404 |doi= 10.1038/nature02055 |display-authors=etal}}
- {{cite journal | vauthors=Ota T, Suzuki Y, Nishikawa T |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 |display-authors=etal}}
- {{cite journal | vauthors=Kakuda S, Oka S, Kawasaki T |title=Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli. |journal=Protein Expr. Purif. |volume=35 |issue= 1 |pages= 111–9 |year= 2004 |pmid= 15039073 |doi= 10.1016/j.pep.2003.12.021 }}
- {{cite journal | vauthors=Kakuda S, Sato Y, Tonoyama Y |title=Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate. |journal=Glycobiology |volume=15 |issue= 2 |pages= 203–10 |year= 2005 |pmid= 15470230 |doi= 10.1093/glycob/cwi001 |display-authors=etal}}
- {{cite journal | vauthors=Kimura K, Wakamatsu A, Suzuki Y |title=Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. |journal=Genome Res. |volume=16 |issue= 1 |pages= 55–65 |year= 2006 |pmid= 16344560 |doi= 10.1101/gr.4039406 | pmc=1356129 |display-authors=etal}}
- {{cite journal | vauthors=Shiba T, Kakuda S, Ishiguro M |title=Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope. |journal=Proteins |volume=65 |issue= 2 |pages= 499–508 |year= 2006 |pmid= 16897771 |doi= 10.1002/prot.21118 |display-authors=etal}}
{{refend}}{{PDB Gallery|geneid=135152}}{{Glycosyltransferases}}{{gene-6-stub}} |