Countess of Shaftesbury is a title given to the wife of the Earl of Shaftesbury. Women who have held the title include:

• Dorothy Ashley-Cooper, Countess of Shaftesbury (c.1656-1698)
• Susanna Ashley-Cooper, Countess of Shaftesbury (1711-1771), first wife of Anthony Ashley Cooper, 4th Earl of Shaftesbury
• Constance Ashley-Cooper, Countess of Shaftesbury (1875-1957), wife of Anthony Ashley-Cooper, 9th Earl of Shaftesbury
• Jamila M'Barek (born c.1961), widow of Anthony Ashley-Cooper, 10th Earl of Shaftesbury

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