| 词条 | ENO3 |
| 释义 |
Enolase 3 (ENO3), more commonly known as beta-enolase (ENO-β), is an enzyme that in humans is encoded by the ENO3 gene. This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have been associated with glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010][1] StructureENO3 is one of three enolase isoforms, the other two being ENO1 (ENO-α) and ENO2 (ENO-γ).[2][3] Each isoform is a protein subunit that can form hetero- or homodimers of the following combinations: αα, αβ, αγ, ββ, and γγ.[4][5] GeneThe ENO3 gene spans 6 kb and contains 12 exons, though the first exon is an untranslated region and, thus, non-coding. This first intron, along with the 5'-flanking region, contains a consensus sequence for muscle-specific regulatory factors that includes a CC(A + T-rich)6GG box, a M-CAT-box CAATCCT, and two myocyte-specific enhancer-binding factor 1 boxes.[3] Upstream of the first exon lies a TATA-like box and CpG-rich region, which contains recognition motifs for binding transcriptional regulatory factors such as Sp1, activator protein 1 and 2, CCAAT box transcription factor/nuclear factor I, and cyclic AMP.[3] Unlike the other enolase genes, which possess multiple transcription initiation sites, ENO3 possesses a single initiation site located 26 bp downstream of the TATA-like box. ProteinThis gene encodes a 433-residue dimeric protein.[3] Due to its comparatively small length and highly conserved intron/exon organization among the three enolase isoforms, ENO3 is suggested to have been the last to diverge from a common ancestral gene. FunctionAs an enolase, ENO3 is a glycolytic enzyme that catalyzes the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate.[3][4] This particular isoform is predominantly expressed in adult striated muscle, including skeletal and cardiac muscle.[2][3] During fetal muscle development, there is a transcriptional switch from expressing ENO1 to ENO3 influenced by muscle innervation and Myo D1.[3] ENO3 is expressed at higher levels in fast-twitch fibers than in slow-twitch fibers. Clinical significanceENO3 has been associated with energy metabolism in cancer cells. TFG-TEC, an oncoprotein, activates ENO3 expression by altering the chromatin structure of the ENO3 promoter and increasing the acetylation of histone H3.[4] Muscle β-enolase deficiency (glycogen storage disease type XIII) is a rare inherited metabolic myopathy caused by a defect in the enzyme's active site, thus disrupting its glycolytic activity. Though this deficiency is characterized as an autosomal recessive condition, both heterozygous and homozygous mutations were identified in the ENO3 gene. The heterozygous mutations were linked to milder symptoms while the homozygous mutations tended to produce more severe symptoms, including rhabdomyolysis. Advances in genetic testing, such as exome sequencing and specific gene panels, can provide greater access to diagnoses for muscle β-enolase deficiency and other rare disorders.[5] InteractionsTFG-TEC binds to the proximal promoter region of the ENO3 gene.[4] Interactive pathway map{{GlycolysisGluconeogenesis_WP534|highlight=ENO3}}See also
References1. ^{{cite web | title = ENO3 enolase 3 (beta, muscle) | url = https://www.ncbi.nlm.nih.gov/gene/2027| accessdate = |work=NCBI Entrez Gene database}} [2][3][4][5]2. ^1 2 3 4 5 6 7 {{cite journal | vauthors = Peshavaria M, Day IN | title = Molecular structure of the human muscle-specific enolase gene (ENO3) | journal = The Biochemical Journal | volume = 275 | pages = 427–33 | date = Apr 1991 | pmid = 1840492 | issue=2 | pmc=1150071 | doi=10.1042/bj2750427}} 3. ^1 2 3 4 {{cite journal | vauthors = Kim AY, Lim B, Choi J, Kim J | title = The TFG-TEC oncoprotein induces transcriptional activation of the human β-enolase gene via chromatin modification of the promoter region | journal = Molecular Carcinogenesis | date = Aug 2015 | pmid = 26310886 | doi = 10.1002/mc.22384 }} 4. ^1 2 {{cite journal | vauthors = Musumeci O, Brady S, Rodolico C, Ciranni A, Montagnese F, Aguennouz M, Kirk R, Allen E, Godfrey R, Romeo S, Murphy E, Rahman S, Quinlivan R, Toscano A | title = Recurrent rhabdomyolysis due to muscle β-enolase deficiency: very rare or underestimated? | journal = Journal of Neurology | volume = 261 | issue = 12 | pages = 2424–8 | date = Dec 2014 | pmid = 25267339 | doi = 10.1007/s00415-014-7512-7 }} 5. ^1 2 {{cite journal | vauthors = Zhu X, Miao X, Wu Y, Li C, Guo Y, Liu Y, Chen Y, Lu X, Wang Y, He S | title = ENO1 promotes tumor proliferation and cell adhesion mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphomas | journal = Experimental Cell Research | volume = 335 | issue = 2 | pages = 216–23 | date = Jul 2015 | pmid = 26024773 | doi = 10.1016/j.yexcr.2015.05.020 }} }} Further reading{{refbegin|33em}}
|
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。