| 词条 | Fluo-4 |
| 释义 |
| ImageFile = Fluo-4-corrected.svg | ImageFile_Ref = {{chemboximage|correct|??}} | ImageSize = 244 | ImageName = Skeletal formula of tetradeprotonated fluo-4 | IUPACName = Fluo-4 | SystematicName = 2,2'-((2-(2-(2-(bis(carboxymethyl)amino)-5-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy)ethoxy)-4-methylphenyl)azanediyl)diacetic acid |Section1={{Chembox Identifiers | MeSHName = Fluo-4 | CASNo = 273221-59-3 | ChEBI = 51103 | PubChem = 25058176 | SMILES = CC1=CC(OCCOC2=CC(=CC=C2N(CC(O)=O)CC(O)=O)C2=C3C=C(F)C(=O)C=C3OC3=C2C=C(F)C(O)=C3)=C(C=C1)N(CC(O)=O)CC(O)=O | StdInChI = 1S/C36H30F2N2O13/c1-18-2-4-20(39(14-32(43)44)15-33(45)46)9-28(18)51-6-7-52-31-8-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-21-10-23(37)26(41)12-29(21)53-30-13-27(42)24(38)11-22(30)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50) | StdInChI_Ref = {{stdinchicite|correct|chemspider}} | StdInChIKey = WZZRKOQVHRRNDH-UHFFFAOYSA-N | StdInChIKey_Ref = {{stdinchicite|correct|chemspider}} }} |Section2={{Chembox Properties | C=36 | H=30 | F=2 | N=2 | O=13 }} }} Fluo-4 is used to measure calcium (Ca2+) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels. The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3. It is commonly used as the non-fluorescent acetoxymethyl ester (Fluo-4 AM) which is cleaved inside the cell to give the free, fluorescent Fluo-4. It loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser which is often used in biological research laboratories.[1] Fluo-4 and its cell-permeable AM ester are available from a few commercial vendors. References1. ^{{cite journal |vauthors=Gee KR, Brown KA, Chen WN, Bishop-Stewart J, Gray D, Johnson I |title=Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes |journal=Cell Calcium |volume=27 |issue=2 |pages=97–106 |date=February 2000 |pmid=10756976 |doi=10.1054/ceca.1999.0095 |url=}} {{Organic-compound-stub}} 8 : Biochemistry methods|Cell imaging|Chelating agents|Fluorone dyes|Fluoroarenes|Acetic acids|Phenol ethers|Anilines |
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