James Downing may refer to:

• James W. Downing (1913–2018), American naval officer
• Jim Downing (born 1942), American racing driver, owner, and developer
• Jim Downing (Gaelic footballer) (1946–2012), Irish Gaelic footballer

• Penikese Island (Massachusetts)
• Penile agenesis and testicular agenesis
• Penile artery
• Penile bone
• Penile cancer
• Penilee
• Penile fracture
• Penile inversion
• Penile length
• Penile plethysmograph
• Penile plethysmography
• Penile subincision
• Penillion
• Penine Way
• Peninsula Campaign
• Peninsula Corridor Joint Powers Board
• Peninsula Expressway, Cape Town
• Peninsula Expressway (Cape Town)
• Peninsula Group
• Peninsula Hotel
• Peninsula Malaysia
• Peninsula of Avalon
• Peninsula, OH
• Peninsula, Ohio
• Peninsula Pilots