This is a list of airlines which have an Air Operator Certificate issued by the Civil Aviation Authority of Colombia.
| Airline | IATA | ICAO | Callsign | IMAGE | Hub Airport(s) | Notes |
|---|
| AerCaribe | ACL | ADMIRE | El Dorado International Airport |
| Aerosucre | 6N | KRE | AEROSUCRE | El Dorado International Airport |
| Aexpa | Matecaña International Airport |
| Air Colombia | La Vanguardia Airport |
| Aliansa - Aerolineas Andinas | La Vanguardia Airport |
| Avianca | AV | AVA | AVIANCA | El Dorado International Airport |
| Avianca Cargo | El Dorado International Airport |
| Central Charter de Colombia | El Dorado International Airport |
| Copa Airlines Colombia | P5 | RPB | AEROREPUBLICA | El Dorado International Airport |
| EasyFly | EF | EFY |
| Heliandes | Enrique Olaya Herrera Airport |
| Helicol | HEL | HELICOL | El Dorado International Airport |
| LATAM Cargo Colombia | L7 | LAE | El Dorado International Airport |
| LATAM Colombia | 4C | ARE | LAN COLOMBIA | El Dorado International Airport | Rebranded from Aires (1981-2011) |
| Líneas Aéreas Suramericanas | LAU | SURAMERICANO | El Dorado International Airport |
| SADELCA | SDK | SADELCA | El Dorado International Airport |
| SAEP | KSP | El Dorado International Airport |
| SARPA | Enrique Olaya Herrera Airport |
| SATENA | 9R | NSE | SATENA | Enrique Olaya Herrera Airport, El Dorado International Airport |
| Searca SA | SRC | SEARCA |
| SELVA - Servicios Aéreos del Vaupes | SDV | SELVA | La Vanguardia Airport |
|
| TAC Colombia | TAC | TCC | TAC | Alfonso Bonilla Aragón International Airport |
| Vertical de Aviación | El Dorado International Airport |
| Viva Air Colombia | 5Z | VVC | VIVA COLOMBIA | Alfonso Bonilla Aragón International Airport, El Dorado International AirportJosé María Córdova International Airport |
| Wingo | P5 | RPB | AEROREPUBLICA | El Dorado International Airport |
|