These are the number-one singles of 1965 according to the Top 100 Singles chart in Cashbox magazine.
| Issue Date | Song | Artist | | January 2 | I Feel Fine | The Beatles |
| January 9 |
| January 16 |
| January 23 | Downtown | Petula Clark |
| January 30 |
| February 6 | You've Lost That Lovin' Feelin' | The Righteous Brothers |
| February 13 |
| February 20 |
| February 27 | This Diamond Ring | Gary Lewis & the Playboys |
| March 6 | Eight Days a Week | The Beatles |
| March 13 |
| March 20 |
| March 27 | Stop! In the Name of Love | The Supremes |
| April 3 | Can't You Hear My Heartbeat | Herman's Hermits |
| April 10 | I'm Telling You Now | Freddie & The Dreamers |
| April 17 |
| April 24 | Game of Love | Wayne Fontana & The Mindbenders |
| May 1 | Mrs. Brown You've Got a Lovely Daughter | Herman's Hermits |
| May 8 |
| May 15 |
| May 22 |
| May 29 | Ticket to Ride | The Beatles |
| June 5 | Back in My Arms Again | The Supremes |
| June 12 | Help Me, Rhonda | The Beach Boys |
| June 19 | I Can't Help Myself (Sugar Pie, Honey Bunch) | The Four Tops |
| June 26 |
| July 3 | Mr. Tambourine Man | The Byrds |
| July 10 | (I Can't Get No) Satisfaction | The Rolling Stones |
| July 17 |
| July 24 |
| July 31 |
| August 7 | I'm Henry VIII, I Am | Herman's Hermits |
| August 14 | I Got You Babe | Sonny & Cher |
| August 21 |
| August 28 | Help! | The Beatles |
| September 4 |
| September 11 |
| September 18 | Like a Rolling Stone | Bob Dylan |
| September 25 | Eve of Destruction | Barry McGuire |
| October 2 | Hang on Sloopy | The McCoys |
| October 9 | Yesterday | The Beatles |
| October 16 |
| October 23 | A Lover's Concerto | The Toys |
| October 30 | Yesterday | The Beatles |
| November 6 | Get Off of My Cloud | The Rolling Stones |
| November 13 |
| November 20 | I Hear a Symphony | The Supremes |
| November 27 | 1-2-3 | Len Barry |
| December 4 | Turn! Turn! Turn! (To Everything There Is a Season) | The Byrds |
| December 11 | Let's Hang On! | The 4 Seasons |
| December 18 | Taste of Honey | Herb Alpert & The Tijuana Brass |
| December 25 | Over and Over | The Dave Clark Five |