“List of restriction enzyme cutting sites”的意思、由来-开放百科全书

A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria. One special kind of restriction enzymes is the class of "homing endonucleases", these being present in all three domains of life, although their function seems to be very different from one domain to another.

The classical restriction enzymes cut up, and hence render harmless, any unknown (non-cellular) DNA that enters a bacterial cell as a result of a viral infection. They recognize a specific DNA sequence, usually short (3 to 8 bp), and cut it, producing either blunt or overhung ends, either at or nearby the recognition site.

Restriction enzymes are quite variable in the short DNA sequences they recognize. An organism often has several different enzymes, each specific to a distinct short DNA sequence.^[1]

Restriction enzymes catalog

The list includes some of the most studied examples of restriction endoncleases. The following information is given:

The whole list contains more than 1,200 enzymes, but databases register about 4,000.^[2]

To make a list that is accessible to navigation, this list has been divided into different pages. Each page contains somewhere between 120-150 entries. Choose a letter to go to a specific part of the list:

nobreak=yes| side=yes| a=|b=| name=Whole list navigation| sep=–| pre1=A | pre1link=List of restriction enzyme cutting sites: A#Whole list navigation| pre2=Ba-Bc | pre2link=List of restriction enzyme cutting sites: Ba-Bc#Whole list navigation| pre3=Bd-Bp | pre3link=List of restriction enzyme cutting sites: Bd-Bp#Whole list navigation| pre4=Bsa-Bso | pre4link=List of restriction enzyme cutting sites: Bsa-Bso#Whole list navigation| pre5=Bsp | pre5link=List of restriction enzyme cutting sites: Bsp-Bss#Whole list navigation| pre6=Bsr-Bss | pre6link=List of restriction enzyme cutting sites: Bsp-Bss#Bsr - Bss| pre7=Bst | pre7link=List of restriction enzyme cutting sites: Bst-Bv#Whole list navigation| pre8=Bsu-Bv | pre8link=List of restriction enzyme cutting sites: Bst-Bv#Bsu - Bv| c=C| d=D| e=E| f=F| g=G| h=H| i=I| k=K| l=L| m=M| n=N| o=O| p=P| r=R| s=S| t=T| u=U| v=V| x=X| y=Y| z=Z| j=|q=|w=| notes=yes| extlinks=yes| seealso=yes}}

Notes and references

1. ^{{cite journal | author = Roberts RJ | title = Restriction and modification enzymes and their recognition sequences | journal = Nucleic Acids Res. | volume = 8 | issue = 1 | pages = r63–r80 |date=January 1980 | pmid = 6243774 | pmc = 327257 | doi = 10.1093/nar/8.1.197-d | url = }}
2. ^{{cite web | url = http://rebase.neb.com | title = REBASE | vauthors = Roberts RJ, Vincze T, Posfai J, Macelis D | date = | work = | publisher = | pages = | archiveurl = | archivedate = | quote = Restriction Enzyme Database. | accessdate = 2010-05-23}}

External links

Databases and lists of restriction enzymes:

Very comprehensive database of restriction enzymes supported by New England Biolabs©. It includes all kind of biological, structural, kinetical and commercial information about thousands of enzymes. Also includes related literature for every molecule: {{cite web | url = http://rebase.neb.com | title = REBASE | vauthors = Roberts RJ, Vincze T, Posfai J, Macelis D | date = | work = | publisher = | pages = | archiveurl = | archivedate = | quote = Restriction Enzyme Database. | accessdate = 2010-05-23}}

Detailed information for biochemical experiments: {{cite web | url = http://www.neb.com/nebecomm/EnzymeFinder.asp? | title = Enzyme finder | quote = New England Biolabs© enzyme finder. | accessdate = 2010-01-07}}

Alphabetical list of enzymes and their restriction sites: {{cite web | url = http://www.genscript.com/cgi-bin/products/enzyme.cgi?op=all_ez | title = GenScript© Restriction Enzyme webpage | accessdate = 2010-01-07}}

General information about restriction sites and biochemical conditions for restriction reactions: {{cite web | url = http://www.promega.com/guides/re_guide/ | title = Restriction Enzymes Resource | quote = Promega© restriction enzymes webpage. | accessdate = 2010-01-07}}

Databases of proteins:

Database of structures of proteins, solved at atomic resolution: {{cite web | url = http://www.wwpdb.org | title = PDB | author = | date = | work = | pages = | archiveurl = | archivedate = | quote = Worldwide Protein Data Bank. | accessdate = 2010-01-25}}

Databases of proteins: {{cite web | url = http://www.expasy.ch/sprot | title = UniProtKB/Swiss-Prot & TrEMBL | author = Swiss Institute of Bioinformatics (SIB) | author2 = European Bioinformatics Institute (EBI) | author-link = Swiss Institute of Bioinformatics | author2-link = European Bioinformatics Institute | last-author-amp = yes | date = | work = | publisher = | pages = | archiveurl = | archivedate = | quote = Swiss-Prot is a curated protein sequence database which strives to provide a high level of annotation (such as the description of the function of a protein, its domains structure, post-translational modifications, variants, etc.), a minimal level of redundancy and high level of integration with other databases. TrEMBL is a computer-annotated supplement of Swiss-Prot that contains all the translations of EMBL nucleotide sequence entries not yet integrated in Swiss-Prot. | accessdate = 2010-01-25}}

5 : Molecular biology|Biotechnology|Restriction enzymes|Enzymes|Proteins

Restriction enzymes catalog

Notes and references

See also

External links