“Lucifer yellow”的意思、由来-开放百科全书

词条

Lucifer yellow

释义

Preparations
References
External links

{{Chembox
| Verifiedfields = changed
| Watchedfields = changed
| verifiedrevid = 410367079
| ImageFile = Lucifer yellow.svg
| ImageSize = 200px
| IUPACName = dilithium 6-Amino-2-(hydrazinecarbonyl)-1,3-dioxobenzo[de]isoquinoline-5,8-disulfonate
| Section1 = {{Chembox Identifiers
| PubChem = 20835957
| ChEBI_Ref = {{ebicite|correct|EBI}}
| ChEBI = 52104
| SMILES = c1c(cc2c3c1c(c(cc3c(=O)n(c2=O)C(=O)NN)S(=O)(=O)[O-])N)S(=O)(=O)[O-].[Li+].[Li+]
| ChemSpiderID_Ref = {{chemspidercite|changed|chemspider}}
| ChemSpiderID = 20137740
| InChI = 1/C13H10N4O9S2.2Li/c14-10-5-1-4(27(21,22)23)2-6-9(5)7(3-8(10)28(24,25)26)12(19)17(11(6)18)13(20)16-15;;/h1-3H,14-15H2,(H,16,20)(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2
| InChIKey = DLBFLQKQABVKGT-NUQVWONBAD
| StdInChI_Ref = {{stdinchicite|changed|chemspider}}
| StdInChI = 1S/C13H10N4O9S2.2Li/c14-10-5-1-4(27(21,22)23)2-6-9(5)7(3-8(10)28(24,25)26)12(19)17(11(6)18)13(20)16-15;;/h1-3H,14-15H2,(H,16,20)(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2
| StdInChIKey_Ref = {{stdinchicite|changed|chemspider}}
| StdInChIKey = DLBFLQKQABVKGT-UHFFFAOYSA-L }}
| Section2 = {{Chembox Properties
|C=13|H=10|N=4|O=9|S=2|Li=2
}}
}}Lucifer yellow is a fluorescent dye used in cell biology.^[1] The key property of Lucifer yellow is that it can be readily visualized in both living and fixed cells using a fluorescence microscope. Lucifer yellow was invented by Walter W. Stewart at the National Institutes of Health and patented in 1978.^[2]

Preparations

For common usage it is compounded with carbohydrazide (CH) and prepared as a lithium salt. The CH group allows it to be covalently linked to surrounding biomolecules during aldehyde fixation.^[3]

Other cations such as ammonium or potassium can be used when lithium is undesirable, but the resulting salts are less soluble in water.

Lucifer yellow can also be compounded as a vinyl sulfone, with ethylenediamine, or with cadaverine. {{what|date=January 2016}}

References

1. ^{{cite journal|last=Hanani|first=Menachem|title=Lucifer yellow – an angel rather than the devil|journal=Journal of Cellular and Molecular Medicine|date=January 2012|volume=16|issue=2|pages=22–31|doi=10.1111/j.1582-4934.2011.01378.x|pmid=21740513|pmc=3823090}}
2. ^Patent description
3. ^{{cite web|title=Lucifer Yellow CH, Lithium Salt|url=http://www.lifetechnologies.com/order/catalog/product/L453|work=Molecular Probes|accessdate=17 March 2014}}

External links

[https://web.archive.org/web/20110516164133/http://probes.invitrogen.com/media/pis/mp00453.pdf Invitrogen's manual for Lucifer yellow]
Molecular structure and spectra of Lucifer yellow CH (lithium salt)

3 : Fluorescent dyes|Hydrazides|Sulfonates

随便看

开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。