1. Composition

2. See also

3. References

4. External links

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis.^[1] It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition

Ingredients (g/L)^[2]

• Ammonium sulfate, 0.50
• Monopotassium phosphate, 1.50
• Disodium phosphate, 1.50
• Sodium citrate, 0.40
• Magnesium sulfate, 0.025
• Zinc sulfate, 0.001
• Copper sulfate, 0.001
• L-Glutamic acid, 0.50
• Ferric ammonium citrate, 0.04
• Pyridoxine hydrochloride, 0.001
• Biotin, 0.0005
• Malachite green, 0.00025
• Agar, 15.00

Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks.

See also

• Lowenstein-Jensen medium
• Middlebrook 7H9 Broth

References

External links

• Middlebrook 7H10 Agar

http://www.bd.com/ds/productCenter/295964.asp

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