| 词条 | Moeller stain | |
| 释义 |
Moeller staining involves the use of a steamed dye reagent in order to increase the stainability of endospores; carbol fuchsin is the primary stain used in this method. Endospores are stained red, while the counterstain methylene blue stains the vegetative bacteria blue. Endospores are surrounded by a highly resistant spore coat, which is highly resistant to excessive heat, freezing, desiccation, as well as chemical agents. More importantly, for identification, spores are resistant to commonly employed staining techniques; therefore alternative staining methods are required. MethodCarbol fuchsin is applied to a heat-fixed slide. The slide is then heated over a bunsen burner, or suspended over a hot water bath, covered with a paper towel, and steamed for 3 minutes. The slide is rinsed with acidified ethanol, and counter-stained with Methylene blue. An improved method involves the addition of the surfactant Tergitol 7 to the carbol fuchsin stain, and the omission of the steaming step.[1] See also
Further reading
References1. ^{{cite journal | pmid = 17933713 | volume=12 | title=Proposal of a simplified technique for staining bacterial spores without applying heat--successful modification of Moeller's method | date=August 2007 | journal=Eur. J. Med. Res. | pages=356–9 | last1 = Hayama | first1 = M | last2 = Oana | first2 = K | last3 = Kozakai | first3 = T |display-authors=etal }} {{microbiology-stub}} 4 : Microbiology techniques|Staining|Bacteriology|Microscopy |
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