The following railroads operate in the U.S. state of Kentucky.
{{TOC right}}| Name | Mark | System[2] | From | To | Successor | Notes | | Altamont and Manchester Railroad | L&N | 1890 | 1898 | Louisville and Nashville Railroad |
| Artemus–Jellico Railroad | AJR | 1924 | 1952 | N/A |
| Ashland Coal and Iron Railway | C&O | 1880 | 1933 | Chesapeake and Ohio Railway |
| Baltimore and Ohio Railroad | B&O
BO | B&O | 1905 | 1987 | Chesapeake and Ohio Railway |
| Bardstown and Louisville Railroad | L&N | 1854 | 1864 | Louisville and Nashville Railroad |
| Barren County Railroad | L&N | 1856 | 1868 | Glasgow Railroad |
| Beattyville and Cumberland Gap Railroad | L&N | 1893 | 1900 | Louisville and Atlantic Railroad |
| Big Sandy Railway | C&O | 1902 | 1906 | Chesapeake and Ohio Railway of Kentucky |
| Big Sandy and Cumberland Railroad | BS&C | N&W | 1900 | 1932 | Norfolk and Western Railway |
| Big Sandy and Kentucky River Railway | BS&K | C&O | 1912 | 1933 | Chesapeake and Ohio Railway |
| Bowling Green and Tennessee Railroad | L&N | 1850 | 1852 | Louisville and Nashville Railroad |
| Bowlinggreen Portage Railway | L&N | 1836 | 1853 | Louisville and Nashville Railroad |
| Brooksville Railroad | 1895 | 1919 | Brooksville and Ohio River Railroad |
| Brooksville and Ohio River Railroad | 1919 | 1931 | N/A |
| Buck Creek Railroad | N&W | 1924? | 1936 | Norfolk and Western Railway |
| Burlington Northern Inc. | BN | 1970 | 1981 | Burlington Northern Railroad |
| Burlington Northern Railroad | BN | 1981 | 1996 | BNSF Railway |
| Burnside and Cincinnati Railway | SOU | 1903 | 1905 | Cincinnati, Burnside and Cumberland River Railway |
| Burnside and Cumberland River Railway | SOU | 1890 | 1905 | Burnside and Cincinnati Railway |
| Cadiz Railroad | CAD | 1901 | 1988 | N/A |
| Caney Valley Railway | 1910 | 1933? | N/A |
| Carolina, Clinchfield and Ohio Railway | CC&O, CCO | ACL
L&N | lk=no|1915}} | 1990 | CSX Transportation |
| Carolina, Cumberland Gap and Chicago Railway | 1882 | 1895 | N/A | Sold at foreclosure; no railroad owned in Kentucky |
| Carrollton and Worthville Railroad | 1905 | 1929 | Carrollton Railroad |
| Central City Passenger and Transportation Company | C&O | 1888 | 1888 | Passenger and Belt Railway |
| Central Mississippi Railroad | IC | 1877 | 1878 | Chicago, St. Louis and New Orleans Railroad |
| Central Transfer Company | L&N
SOU | 1884 | 1890 | Central Transfer Railway and Storage Company |
| Central Transfer Railway and Storage Company | L&N
SOU | 1890 | Still exists as a joint subsidiary of CSX Transportation and the Norfolk Southern Railway |
| Charleston, Cincinnati and Chicago Railroad | C&O | 1887 | 1893 | Ohio River and Charleston Railway of Kentucky |
| Chattaroi Railway | C&O | 1873 | 1888 | Ohio and Big Sandy Railroad |
| Chesapeake and Nashville Railway | L&N | 1884 | 1892 | Gallatin and Scottsville Railway |
| Chesapeake and Ohio Railway | C&O
CO | C&O | lk=no|1880}} | 1987 | CSX Transportation |
| Chesapeake and Ohio Railway of Kentucky | C&O | 1904 | 1907 | Chesapeake and Ohio Railway |
| Chesapeake and Ohio Northern Railway | C&O | 1914 | 1930 | Chesapeake and Ohio Railway |
| Chesapeake, Ohio and Southwestern Railroad | IC | 1882 | 1896 | Chicago, St. Louis and New Orleans Railroad |
| Chicago, Burlington and Quincy Railroad | CB&Q
CBQ | CB&Q | ? | 1970 | Burlington Northern Inc. |
| Chicago, Indianapolis and Louisville Railway | CI&L
CIL | MON | 1897 | 1956 | Monon Railroad |
| Chicago, Memphis and Gulf Railroad | IC | 1910 | 1963 | Illinois Central Railroad |
| Chicago, Milwaukee, St. Paul and Pacific Railroad | MILW | MILW | 1973 | 1985 | The Milwaukee Road, Inc. |
| Chicago, St. Louis and New Orleans Railroad | IC | 1878 | 1951 | Illinois Central Railroad |
| Cincinnati, Burnside and Cumberland River Railway | SOU | 1905 | 1970 | Cincinnati, New Orleans and Texas Pacific Railway |
| Cincinnati, Flemingsburg and Southeastern Railroad | 1905 | 1920? | Flemingsburg and Northern Railroad |
| Cincinnati, Green River and Nashville Railroad | L&N | 1882 | 1884 | Chesapeake and Nashville Railway |
| Cincinnati, Lexington and East Tennessee Railroad | SOU | 1867 | 1873 | Cincinnati Southern Railway |
| Cincinnati and Southeastern Railway | 1880 | 1892? | Covington, Flemingsburg and Ashland Railway |
| Cincinnati Southern Railway | SOU | 1869 | Still exists as a lessor of Norfolk Southern Railway operating subsidiary Cincinnati, New Orleans and Texas Pacific Railway |
| City Passenger Railway | C&O | 1886 | 1888 | Central City Passenger and Transportation Company |
| Clinchfield Railroad | CRR | ACL
L&N | 1924 | 1983 | Seaboard System Railroad |
| Clinchfield Northern Railway of Kentucky | ACL
L&N | 1911 | 1940 | Carolina, Clinchfield and Ohio Railway |
| Consolidated Rail Corporation | CR | 1976 | 1994 | Louisville and Indiana Railroad |
| Covington and Cincinnati Elevated Railroad and Transfer and Bridge Company | C&O | 1886 | 1985 | Chesapeake and Ohio Railway |
| Covington and Cincinnati Pier Bridge Company | C&O | 1884 | 1886 | Covington and Cincinnati Elevated Railroad and Transfer and Bridge Company |
| Covington, Flemingsburg and Ashland Railway | 1892 | 1905 | Cincinnati, Flemingsburg and Southeastern Railroad |
| Covington, Flemingsburg and Pound Gap Railway | 1876 | 1879 | Licking Valley Railway |
| Covington, Flemingsburg and Southeastern Railway | 1880 | 1880 | Cincinnati and Southeastern Railway |
| Covington and Lexington Railroad | L&N | 1849 | 1859 | Kentucky Central Railroad |
| Covington Short Route Transfer Railway | C&O | 1888 | 1904 | Chesapeake and Ohio Railway of Kentucky |
| Cumberland Railroad | CMB | 1902 | 1924 | Artemus–Jellico Railroad |
| Cumberland Railway | SOU | 1905? | 1944? | Southern Railway |
| Cumberland Railway | 1882 | 1882 | Carolina, Cumberland Gap and Chicago Railway |
| Cumberland and Manchester Railroad | C&M | L&N | 1915 | 1936 | Louisville and Nashville Railroad |
| Cumberland and Ohio Railroad | L&N | 1869 | 1878 | Northern Division of the Cumberland and Ohio Railroad
Southern Division of the Cumberland and Ohio Railroad |
| Cumberland River and Tennessee Railroad | L&N | 1893 | 1901 | Louisville and Nashville Railroad |
| East Kentucky Southern Railway | EKS | 1928 | 1933 | N/A |
| East Tennessee, Virginia and Georgia Railway | SOU | 1890? | 1894 | Southern Railway |
| Eastern Kentucky Railway | EK | 1870 | 1929 | East Kentucky Southern Railway |
| Elizabethtown, Lexington and Big Sandy Railroad | C&O | 1869 | 1902 | Lexington and Big Sandy Railway |
| Elizabethtown and Paducah Railroad | IC | 1867 | 1874 | Louisville, Paducah and Southwestern Railroad |
| Elkhorn and Beaver Valley Railway | C&O | 1904 | Chesapeake and Ohio Railway |
| Elkhorn Southern Railway | ACL
L&N | 1906 | 1911 | Clinchfield Northern Railway of Kentucky |
| Elkton Railroad | L&N | 1871 | 1884 | Elkton and Guthrie Railroad |
| Elkton and Guthrie Railroad | L&N | 1884 | 1957 | N/A |
| Evansville, Henderson and Nashville Railroad | L&N | 1867 | 1872 | St. Louis and South Eastern Railway |
| Evansville, Owensboro and Nashville Railroad | L&N | 1873 | 1877 | Owensboro and Nashville Railroad |
| Falls City Belt Line Railway | PRR | 1902 | 1903 | Pennsylvania Terminal Railway |
| Flemingsburg and Northern Railroad | 1920 | 1955 | N/A |
| Frankfort and Cincinnati Railroad | FCIN | 1927 | 1985 | N/A |
| Frankfort and Cincinnati Railway | 1897 | 1927 | Frankfort and Cincinnati Railroad |
| Frankfort, Paris and Big Sandy Railroad | 1871 | 1881 | Paris, Georgetown and Frankfort Railroad |
| Gallatin and Scottsville Railway | L&N | 1906 | 1907 | Louisville and Nashville Railroad |
| Glasgow Railroad | L&N | 1868 | 1899 | Glasgow Railway |
| Glasgow Railway | L&N | 1899 | Still exists as a lessor of CSX Transportation |
| Gulf, Mobile and Northern Railroad | GM&N | GM&O | 1926 | 1938 | N/A |
| Gulf, Mobile and Ohio Railroad | GM&O
GMO | GM&O | 1940 | 1972 | Illinois Central Gulf Railroad |
| Hardin Southern Railroad | HSRR | 1993 | 2005 | KWT Railway |
| Henderson Belt Railroad | L&N | 1888 | 1957 | Louisville and Nashville Railroad |
| Henderson Bridge Company | L&N | 1872 | 1906 | Henderson Bridge and Railroad Company |
| Henderson Bridge and Railroad Company | L&N | 1906 | 1906 | Louisville and Nashville Railroad |
| Henderson and Nashville Railroad | L&N | 1837 | 1867 | Evansville, Henderson and Nashville Railroad |
| Hickman and Obion Railroad | L&N | 1854 | 1855 | Nashville and North Western Railroad |
| Hodgenville and Elizabethtown Railway | IC | 1884 | 1902 | Chicago, St. Louis and New Orleans Railroad |
| Illinois Central Railroad | IC | IC | 1882? | 1972 | Illinois Central Gulf Railroad |
| Illinois Central Gulf Railroad | ICG | 1972 | 1988 | Illinois Central Railroad |
| Indiana, Alabama and Texas Railroad | L&N | 1885 | 1887 | Louisville and Nashville Railroad |
| Indiana Hi-Rail Corporation | IHRC | 1986 | 1996 | CSX Transportation |
| Jellico, Bird-Eye and Northern Railway | L&N | 1893 | 1902 | Louisville and Nashville Railroad |
| J&J Railroad | JJRD | 1983 | 1993 | Hardin Southern Railroad |
| Kentucky Central Railroad | L&N | 1861 | 1887 | Kentucky Central Railway |
| Kentucky Central Railway | L&N | 1887 | 1891 | Louisville and Nashville Railroad |
| Kentucky Highlands Railroad | L&N | 1907 | 1915 | Louisville and Nashville Railroad |
| Kentucky and Indiana Bridge Company | SOU | 1880 | 1900 | Kentucky and Indiana Bridge and Railroad Company |
| Kentucky and Indiana Bridge and Railroad Company | SOU | 1900 | 1910 | Kentucky and Indiana Terminal Railroad |
| Kentucky and Indiana Terminal Railroad | KIT | SOU | 1910 | 1981 | Southern Railway |
| Kentucky Midland Railroad | IC | 1904 | 1922 | Chicago, St. Louis and New Orleans Railroad |
| Kentucky Midland Railway | 1888 | 1897 | Frankfort and Cincinnati Railway |
| Kentucky Northern Railroad | 1896 | 1900 | N/A |
| Kentucky, Rockcastle and Cumberland Railroad | 1913 | N/A |
| Kentucky and South Atlantic Railroad | C&O | 1888 | 1902 | Lexington and Big Sandy Railway |
| Kentucky and South Atlantic Railway | C&O | 1882 | 1887 | Kentucky and South Atlantic Railroad |
| Kentucky Southern Railroad | L&N | 1872 | 1882 | Louisville, St. Louis and Texas Railway |
| Kentucky and Tennessee Railroad | GM&O | 1870 | 1872 | Mobile and Ohio Railroad |
| Kentucky and Tennessee Railroad | 1902 | 1904 | Kentucky and Tennessee Railway |
| Kentucky Union Railway | L&N | 1854 | 1894 | Lexington and Eastern Railway |
| Kentucky Valley Railroad | IC | 1905 | 1913 | Chicago, St. Louis and New Orleans Railroad |
| Kentucky and Virginia Railroad | L&N | 1915 | 1915 | Louisville and Nashville Railroad |
| Kentucky Western Railway | IC | 1899 | 1902 | Chicago, St. Louis and New Orleans Railroad |
| Kinniconnick and Freestone Railway | C&O | 1890 | 1908 | Chesapeake and Ohio Railway of Kentucky |
| Knox Creek Railway | N&W | 1912 | 1936 | Norfolk and Western Railway |
| Knoxville, Cumberland Gap and Louisville Railroad | L&N | 1888 | 1895 | Knoxville, Cumberland Gap and Louisville Railway |
| Knoxville, Cumberland Gap and Louisville Railway | L&N | 1895 | 1896 | Middlesborough Railroad |
| Lenox Railroad | 1918 | 1926? | N/A |
| Levisa River Railroad | C&O | 1911 | 1946? | Chesapeake and Ohio Railway |
| Lexington and Big Sandy Railroad | C&O | 1852 | 1864 | Lexington and Big Sandy Railroad, Eastern Division
Lexington and Big Sandy Railroad, Western Division |
| Lexington and Big Sandy Railroad, Eastern Division | C&O | 1865 | 1880 | Ashland Coal and Iron Railway |
| Lexington and Big Sandy Railroad, Western Division | C&O | 1865 | 1871 | Elizabethtown, Lexington and Big Sandy Railroad |
| Lexington and Big Sandy Railway | C&O | 1902 | 1904 | Chesapeake and Ohio Railway of Kentucky |
| Lexington and Danville Railroad | SOU | 1850 | 1858 | Lexington and Southern Kentucky Railroad |
| Lexington and Eastern Railway | L&N | 1894 | 1915 | Louisville and Nashville Railroad |
| Lexington and Frankfort Railroad | L&N | 1848 | 1869 | Louisville, Cincinnati and Lexington Railroad |
| Lexington and Ohio Railroad | LXOH | 1996 | 2003 | R.J. Corman Railroad/Central Kentucky Lines |
| Lexington and Ohio Railroad | L&N | 1830 | 1842 | Lexington and Frankfort Railroad
Louisville and Frankfort Railroad |
| Lexington and Southern Kentucky Railroad | SOU | 1860 | 1867 | Cincinnati, Lexington and East Tennessee Railroad |
| Lexington Union Station Company | C&O
L&N | 1905 |
| Licking and Lexington Railroad | L&N | 1847 | 1849 | Covington and Lexington Railroad |
| Licking River Railroad | 1899 | 1913 | N/A |
| Licking Valley Railway | 1896 | 1899 | Licking River Railroad |
| Licking Valley Railway | 1880 | 1880 | Covington, Flemingsburg and Southeastern Railway |
| Long Fork Railway | C&O | 1912 | 1933 | Chesapeake and Ohio Railway |
| Louisville and Atlantic Railroad | L&N | 1899 | 1909 | Louisville and Nashville Railroad |
| Louisville Bridge Company | PRR | 1856 | 1918 | Louisville Bridge and Railroad Company |
| Louisville Bridge and Railroad Company | PRR | 1918 | 1918 | Louisville Bridge and Terminal Railway |
| Louisville Bridge and Terminal Railway | PRR | 1918 | 1954 | Penndel Company |
| Louisville, Cincinnati and Lexington Railroad | L&N | 1869 | 1877 | Louisville, Cincinnati and Lexington Railway |
| Louisville, Cincinnati and Lexington Railway | L&N | 1877 | 1881 | Louisville and Nashville Railroad |
| Louisville, Cloverport and Western Railway | L&N | 1880 | 1882 | Louisville, St. Louis and Texas Railway |
| Louisville and Frankfort Railroad | L&N | 1847 | 1869 | Louisville, Cincinnati and Lexington Railroad |
| Louisville, Hardinsburg and Western Railway | L&N | 1888 | 1892 | Louisville, St. Louis and Texas Railway |
| Louisville, Harrodsburg and Virginia Railroad | 1868 | 1884 | Louisville Southern Railroad | Chartered, but never laid track until reörganization |
| Louisville, Harrods Creek and Westport Railroad | L&N | 1879 | 1904 | Louisville and Nashville Railroad |
| Louisville, Harrods Creek and Westport Railway | L&N | 1870 | 1879 | Louisville, Harrods Creek and Westport Railroad |
| Louisville, Henderson and St. Louis Railway | LH&S
LHS | L&N | 1896 | 1984 | Seaboard System Railroad |
| Louisville and Jeffersonville Bridge Company | NYC | 1888 | 1917 | Louisville and Jeffersonville Bridge and Railroad Company |
| Louisville and Jeffersonville Bridge and Railroad Company | NYC | 1917 | 1968 | N/A |
| Louisville and Nashville Railroad | L&N
LN | L&N | 1850 | 1983 | Seaboard System Railroad |
| Louisville, New Albany and Chicago Railway | MON | 1882 | 1897 | Chicago, Indianapolis and Louisville Railway |
| Louisville, Paducah and Southwestern Railroad | IC | 1874 | 1876 | Paducah and Elizabethtown Railroad |
| Louisville Railway Transfer Company | L&N | 1871 | 1893 | Louisville and Nashville Railroad |
| Louisville, St. Louis and Texas Railway | L&N | 1882 | 1896 | Louisville, Henderson and St. Louis Railway |
| Louisville Southern Railroad | LS | SOU | 1884 | 1894 | Southern Railway in Kentucky |
| Louisville and Southwestern Railway | SOU | 1882 | 1889 | Louisville Southern Railroad |
| Madisonville, Hartford and Eastern Railroad | L&N | 1905 | 1912 | Louisville and Nashville Railroad |
| Madisonville and Shawneetown Straight Line Railroad | L&N | 1870 | 1872 | St. Louis and Southeastern Railway |
| Mammoth Cave Railroad | 1874 | 1931 | N/A |
| Maysville and Big Sandy Railroad | C&O | 1850 | 1904 | Chesapeake and Ohio Railway of Kentucky |
| Maysville and Lexington Railroad | L&N | 1850 | 1856 | Maysville and Lexington Railroad, Northern Division
Maysville and Lexington Railroad, Southern Division |
| Maysville and Lexington Railroad, North Division | L&N | 1876 | 1921 | Louisville and Nashville Railroad |
| Maysville and Lexington Railroad, Northern Division | L&N | 1868 | 1875 | Maysville and Lexington Railroad, North Division |
| Maysville and Lexington Railroad, Southern Division | L&N | 1868 | 1921 | Louisville and Nashville Railroad |
| Memphis, Paducah and Northern Railroad | IC | 1878 | 1881 | Chesapeake, Ohio and Southwestern Railroad |
| Middle Division of the Cumberland and Ohio Railroad | L&N | 1880 | 1888 | Louisville and Nashville Railroad |
| Middlesborough Railroad | L&N | 1895 | 1896 | Louisville and Nashville Railroad |
| Middlesborough Belt Railroad | L&N | 1890 | 1895 | Middlesborough Railroad |
| Middlesboro Mineral Railway | SOU | 1900 | 1905 | Cumberland Railway |
| Millers Creek Railroad | C&O | 1909 | 1933 | Chesapeake and Ohio Railway |
| The Milwaukee Road, Inc. | MILW | MILW | 1985 | 1986 | Soo Line Railroad |
| Mississippi Central Railroad | IC | 1859 | 1874 | New Orleans, St. Louis and Chicago Railroad |
| Mississippi River Railway | IC | 1860 | 1872 | Paducah and Memphis Railroad |
| Mobile and Ohio Railroad | GM&O | 1848 | 1940 | Gulf, Mobile and Ohio Railroad |
| Monon Railroad | MON | MON | 1956 | 1971 | Louisville and Nashville Railroad |
| Morehead and North Fork Railroad | 1905 | 1973? | N/A |
| Morehead and West Liberty Railroad | 1906 | Morehead and North Fork Railroad |
| Morganfield and Atlanta Railroad | L&N | 1905 | 1921 | Louisville and Nashville Railroad |
| Mount Sterling Coal Road | C&O | 1874 | 1882 | Kentucky and South Atlantic Railway |
| Mountain Central Railway | 1914 | 1928 | N/A |
| Nashville and Chattanooga Railroad | L&N | 1873 | Nashville, Chattanooga and St. Louis Railway |
| Nashville and Clarksville Railroad | IC | 1901 | 1902 | Tennessee Central Railroad |
| Nashville, Chattanooga and St. Louis Railway | N&C
NC | L&N | 1873 | 1957 | Louisville and Nashville Railroad |
| Nashville and North Western Railroad | L&N | 1855 | 1871 | Nashville and Chattanooga Railroad |
| New Orleans and Ohio Railroad | IC | 1852 | 1869 | Paducah and Gulf Railroad |
| New Orleans, St. Louis and Chicago Railroad | IC | 1874 | 1877 | Central Mississippi Railroad |
| Newport and Cincinnati Bridge Company | L&N | 1868 | 1904 | Louisville and Nashville Railroad |
| Newport News and Mississippi Valley Company | C&O | Chesapeake and Ohio Railway |
| Norfolk and Western Railway | N&W
NW | N&W | 1998 | Norfolk Southern Railway |
| Northern Division of the Cumberland and Ohio Railroad | L&N | 1878 | 1900 | Shelbyville, Bloomfield and Ohio Railroad |
| Ohio and Big Sandy Railroad | C&O | 1889 | 1902 | Lexington and Big Sandy Railway |
| Ohio and Kentucky Railway | 1894 | 1933 | N/A |
| Ohio and Kentucky Bridge Company | C&O | 1886 | 1886 | Covington and Cincinnati Elevated Railroad and Transfer and Bridge Company |
| Ohio, Kentucky and Virginia Railway | C&O | 1886 | 1887 | Charleston, Cincinnati and Chicago Railroad |
| Ohio River and Charleston Railway of Kentucky | C&O | 1894 | 1902 | Lexington and Big Sandy Railway |
| Ohio Valley Railroad | IC | 1880 | 1886 | Ohio Valley Railway |
| Ohio Valley Railway | IC | 1886 | 1897 | Chicago, St. Louis and New Orleans Railroad |
| Ohio Valley Coal and Mining Company | IC | 1886 | 1888 | Ohio Valley Railway |
| Ohio Valley Railroad and Mining Company | IC | 1882 | 1886 | Ohio Valley Coal and Mining Company |
| Olympia and Owingsville Railway | 1916 | 1918 | N/A |
| Owensboro, Falls of Rough and Green River Railroad | IC | 1882 | 1897 | Chicago, St. Louis and New Orleans Railroad |
| Owensboro and Nashville Railroad | L&N | 1877 | 1881 | Owensboro and Nashville Railway |
| Owensboro and Nashville Railway | L&N | 1881 | 1921 | Louisville and Nashville Railroad |
| Owensboro and Russellville Railroad | L&N | 1867 | 1873 | Evansville, Owensboro and Nashville Railroad |
| Owingsville and Olympia Railroad | 1915? | 1916 | Olympia and Owingsville Railway |
| Paducah and Elizabethtown Railroad | IC | 1877 | 1882 | Chesapeake, Ohio and Southwestern Railroad |
| Paducah and Gulf Railroad | IC | 1869 | 1872 | Paducah and Memphis Railroad |
| Paducah and Illinois Railroad | PI | CB&Q
IC
L&N | 1910 | Still exists as a joint subsidiary of the BNSF Railway, Canadian National Railway, and CSX Transportation |
| Paducah and Memphis Railroad | IC | 1872 | 1877 | Memphis, Paducah and Northern Railroad |
| Paducah, Tennessee and Alabama Railroad | L&N | 1892 | 1895 | Louisville and Nashville Railroad |
| Paducah Union Depot Company | IC | 1888 | 1913 | Chicago, St. Louis and New Orleans Railroad |
| Paris, Georgetown and Frankfort Railroad | 1871 | 1888 | Kentucky Midland Railway |
| Passenger and Belt Railway | C&O | 1888 | 1895 | Chesapeake and Ohio Railway |
| Penn Central Transportation Company | PC | 1968 | 1976 | Consolidated Rail Corporation |
| Penndel Company | PRR | 1954 | 1976 | Consolidated Rail Corporation |
| Pennsylvania Railroad | PRR | PRR | 1921? | 1968 | Penn Central Transportation Company |
| Pennsylvania Terminal Railway | PRR | 1903 | 1918 | Louisville Bridge and Terminal Railway |
| Pine Mountain Railroad | L&N | 1905 | 1913 | Louisville and Nashville Railroad |
| Portsmouth and Tygart Valley Railroad | 1892? | ≤1911 | N/A |
| Princeton and Ohio River Railroad | L&N | 1882 | 1885 | Indiana, Alabama and Texas Railroad |
| Red River Valley Railroad | 1914? | N/A |
| Richmond, Nicholasville, Irvine and Beattyville Railroad | L&N | 1888 | 1899 | Louisville and Atlantic Railroad |
| Rockcastle Railroad | 1913 | 1913 | Rockcastle River Railway |
| Rockcastle River Railway | 1913 | 1932 | N/A |
| St. Louis and South Eastern Railway | L&N | 1872 | 1879 | Louisville and Nashville Railroad |
| St. Louis and Southeastern Railway | L&N | 1872 | 1872 | St. Louis and South Eastern Railway |
| Sandy Valley and Elkhorn Railway | C&O | 1902 | 1933 | Chesapeake and Ohio Railway |
| Seaboard System Railroad | SBD | 1983 | 1986 | CSX Transportation |
| Shelby Railroad | L&N | 1851 | 1902 | Louisville and Nashville Railroad |
| Shelbyville, Bloomfield and Ohio Railroad | L&N | 1900 | 1901 | Louisville and Nashville Railroad |
| Short Route Railway Transfer Company | IC | 1873 | 1897 | Chicago, St. Louis and New Orleans Railroad |
| Soo Line Railroad | SOO | 1986 | 2006 | Indiana Rail Road |
| South Kentucky Railroad | IC | 1871 | 1880 | Ohio Valley Railroad |
| South and Western Railroad | ACL
L&N | 1905 | 1908 | Carolina, Clinchfield and Ohio Railway |
| South and Western Railway | ACL
L&N | 1902 | 1906 | South and Western Railroad |
| Southern Railway | SOU | SOU | 1894 | 1990 | Norfolk Southern Railway |
| Southern Railway in Kentucky | SOU | 1894 | 1944? | Southern Railway |
| Southern Division of the Cumberland and Ohio Railroad | L&N | 1878 | 1903 | Louisville and Nashville Railroad |
| Southwestern Railroad | SOU | 1876 | 1889 | Louisville Southern Railroad |
| Tennessee Central Railroad | IC | 1902 | 1922 | Tennessee Central Railway |
| Tennessee Central Railway | TC | IC | 1922 | 1968 | Illinois Central Railroad |
| Tennessee and Kentucky Railroad | L&N | 1881 | 1881 | Owensboro and Nashville Railway |
| Tradewater Railway | TRWY | 1982 | 1995 | Western Kentucky Railway |
| Tug River and Kentucky Railroad | N&W | 1913 | 1936 | Norfolk and Western Railway |
| Versailles and Midway Railway | SOU | 1884 | 1889 | Louisville Southern Railroad |
| Wasioto and Black Mountain Railroad | L&N | 1908 | 1915 | Kentucky and Virginia Railroad |
| Williamson and Pond Creek Railroad | N&W | 1912 | 1936 | Norfolk and Western Railway |
| Winchester and Beattyville Railroad | L&N | 1878 | 1893 | Beattyville and Cumberland Gap Railroad |
| Woodford Railroad | SOU | 1871 | 1889 | Louisville Southern Railroad |
1. ^{{cite web|url=http://www.candoltd.com/rail-operation.html |title=Archived copy |accessdate=2007-05-17 |deadurl=yes |archiveurl=https://web.archive.org/web/20100525043714/http://www.candoltd.com/rail-operation.html |archivedate=2010-05-25 |df= }}
2. ^This is one or more of the Class I railroads that the railroad became part of, if any.