| 词条 | Nepenthesin |
| 释义 |
| Name = Nepenthesin | EC_number = 3.4.23.12 | CAS_number = 9073-80-7 | IUBMB_EC_number = 3/4/23/12 | GO_code = | image = | width = | caption = }}Nepenthesin (also spelled nepenthacin[1][2] or nepenthasin[3]) is an aspartic protease of plant origin that has so far been identified in the pitcher secretions of Nepenthes and in the leaves of Drosera peltata.[4][5][6][7][8][9] It is similar to pepsin, but differs in that it also cleaves on either side of Asp residues and at Lys┼Arg.[3] While more pH and temperature stable than porcine pepsin A, it is considerably less stable in urea or guanidine hydrochloride.[11] It is the only known protein with such a stability profile.[11] The name nepenthesin was coined in 1968 by Shigeru Nakayama and Shizuko Amagase.[13] Alternative names for this enzyme include Nepenthes acid proteinase and Nepenthes aspartic proteinase.[3] Two isozymes have been identified in Nepenthes: nepenthesin I and nepenthesin II.[15] The production of large quantities of nepenthesin-1 through heterologous expression in Escherichia coli was described in 2014. The names cephalotusin, dionaeasin and droserasin have been proposed for similar aspartic endopeptidases originating from the carnivorous plant genera Cephalotus, Dionaea and Drosera, respectively.[17] References1. ^1 {{cite journal | author = Jentsch J | title = Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin | journal = FEBS Lett. | volume = 21 | issue = 3 | pages = 273–276 |date=April 1972 | pmid = 11946525 | doi = 10.1016/0014-5793(72)80181-9 }} [1][2][3][4][5][6][7][8][9][10][11][12][13]2. ^1 {{cite journal |vauthors=Jentsch J, Meierkord S, Hammer M | title = The enzymes from carnivorous plants (Nepenthes): Properties and characterization of the acid protease nepenthacin | journal=Planta Medica | year = 1989 | volume = 55 | pages = 227 | doi = 10.1055/s-2006-961979 | issue = 2 }} 3. ^1 2 3 EC 3.4.23.12 - Nepenthesin. Integrated Enzyme Database (IntEnz). 4. ^1 {{cite journal |vauthors=Amagase S, Nakayama S, Tsugita A | title = Acid protease in Nepenthes. II. Study on the specificity of nepenthesin | journal = J. Biochem. | volume = 66 | issue = 4 | pages = 431–9 |date=October 1969 | pmid = 5354017 | url = http://jb.oxfordjournals.org/cgi/content/abstract/66/4/431 }} 5. ^1 {{cite journal | author = Amagase S | title = Digestive enzymes in insectivorous plants. 3. Acid proteases in the genus Nepenthes and Drosera peltata | journal = J. Biochem. | volume = 72 | issue = 1 | pages = 73–81 |date=July 1972 | pmid = 5069751 | url = http://jb.oxfordjournals.org/cgi/content/abstract/72/1/73}} 6. ^1 {{cite journal |vauthors=Amagase S, Mori M, Nakayama S | title = Digestive enzymes in insectivorous plants. IV. Enzymatic digestion of insects by Nepenthes secretion and Drosera peltata extract: proteolytic and chitinolytic activities | journal = J. Biochem. | volume = 72 | issue = 3 | pages = 765–7 |date=September 1972 | pmid = 4634982 }} 7. ^1 {{ cite journal |vauthors=Tökés ZA, Woon WC, Chambers SM |title=Digestive enzymes secreted by the carnivorous plant Nepenthes macferlanei L | journal = Planta |date=March 1974 | volume = 119 | issue = 1 | pages = 39–46 | doi=10.1007/BF00390820 }} 8. ^1 {{cite book | editor = James, Michael | title = Aspartic proteinases: retroviral and cellular enzymes | edition = | language = | publisher = Plenum | location = New York | year = 1998 |vauthors=Athauda SB, Inoue H, Iwamatsu A, Takahashi K | chapter = Acid Proteinase from Nepenthes distillatoria (Badura) | pages = 453–458 | quote = | isbn = 0-306-45809-8 }} 9. ^1 {{cite journal |vauthors=Takahashi K, Tanji M, Shibata C | title = Variations in the content and isozymic composition of nepenthesin in the pitcher fluids among Nepenthes species | journal = Carnivorous Plant Newsletter | year = 2007 | volume = 36 | issue = 3 | pages = 73–76 | url=http://www.carnivorousplants.org/cpn/articles/CPNv36n3p73_76.pdf }} 10. ^1 {{cite journal |vauthors=Takahashi K, Nishii W, Shibata C | title = The digestive fluid of Drosera indica contains a cysteine endopeptidase ("droserain") similar to dionain from Dionaea muscipula | journal = Carnivorous Plant Newsletter | year = 2012 | volume = 41 | issue = 4 | pages = 132–134 | url= }} 11. ^1 {{cite journal |vauthors=Athauda SB, Matsumoto K, Rajapakshe S, Kuribayashi M, Kojima M, Kubomura-Yoshida N, Iwamatsu A, Shibata C, Inoue H, Takahashi K | title = Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases | journal = Biochem. J. | volume = 381 | issue = Pt 1 | pages = 295–306 |date=July 2004 | pmid = 15035659 | pmc = 1133788 | doi = 10.1042/BJ20031575 }} 12. ^1 2 {{cite journal |vauthors=Kubota K, Metoki Y, Athauda SB, Shibata C, Takahashi K | title = Stability Profiles of Nepenthesin in Urea and Guanidine Hydrochloride: Comparison with Porcine Pepsin A | journal = Bioscience, Biotechnology, and Biochemistry | year = 2010 | volume = 74 | issue = 11 | pages = 2323–2326 | doi = 10.1271/bbb.100391 }} 13. ^1 {{cite journal | vauthors = Nakayama S, Amagase S | title = Acid Protease in Nepenthes: Partial Purification and Properties of the Enzyme | journal = Proceedings of the Japan Academy | year = 1968 | volume = 44 | issue = 5 | pages = 358–362 | url = http://www.journalarchive.jst.go.jp/jnlpdf.php?cdjournal=pjab1945&cdvol=44&noissue=5&startpage=358&lang=en&from=jnlabstract }}{{dead link|date=February 2018 |bot=InternetArchiveBot |fix-attempted=yes }} }} External links
1 : EC 3.4.23 |
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