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词条 Nucleic acid methods
释义

  1. Purification

  2. Quantification

  3. Synthesis

  4. Kinetics

  5. Gene function

  6. Other

  7. See also

  8. References

  9. External links

{{for|protein methods|Protein methods}}

Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Purification

  • Phenol–chloroform extraction
  • Minicolumn purification
  • RNA extraction
  • Boom method
  • Synchronous coefficient of drag alteration (SCODA) DNA purification

Quantification

  • Abundance in weight: spectroscopic nucleic acid quantitation
  • Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
  • High-throughput relative abundance: DNA microarray
  • High-throughput absolute abundance: serial analysis of gene expression (SAGE)
  • Size: gel electrophoresis

Synthesis

  • De novo: oligonucleotide synthesis
  • Amplification: polymerase chain reaction (PCR)

Kinetics

  • Multi-parametric surface plasmon resonance[1][2]
  • Dual-polarization interferometry[3]
  • Quartz crystal microbalance with dissipation monitoring (QCM-D)[4]

Gene function

  • RNA interference

Other

  • Bisulfite sequencing
  • DNA sequencing
  • Expression cloning
  • Fluorescence in situ hybridization
  • Lab-on-a-chip
  • Comparison of nucleic acid simulation software
  • Northern blot
  • Nuclear run-on assay
  • Radioactivity in the life sciences
  • Southern blot
  • Differential centrifugation (sucrose gradient)
  • Toeprinting assay
  • Several bioinformatics methods, as seen in list of RNA structure prediction software

See also

  • CSH Protocols
  • Current Protocols

References

1. ^{{cite journal|last1=Tang|first1=Wei|last2=Hu|first2=Shichao|last3=Wang|first3=Huaming|last4=Zhao|first4=Yan|last5=Li|first5=Na|last6=Liu|first6=Feng|title=A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations|journal=Chem. Commun.|date=23 September 2014|volume=50|issue=92|pages=14352–14355|doi=10.1039/C4CC07041K}}
2. ^{{cite journal|last1=Ihalainen|first1=Petri|last2=Pettersson|first2=Fredrik|last3=Pesonen|first3=Markus|last4=Viitala|first4=Tapani|last5=Määttänen|first5=Anni|last6=Österbacka|first6=Ronald|last7=Peltonen|first7=Jouko|title=An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes|journal=Nanotechnology|date=7 March 2014|volume=25|issue=9|pages=094009|doi=10.1088/0957-4484/25/9/094009}}
3. ^{{Cite journal |last1= Berney |first1= H. |last2= Oliver |first2= K. |doi= 10.1016/j.bios.2004.12.024 |title= Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation |journal= Biosensors and Bioelectronics |volume= 21 |issue= 4 |pages= 618–626 |year= 2005 |pmid= 16202875| pmc= }}
4. ^{{cite journal|last=Dixon|first=Matthew C.|title=Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions|journal=Journal of Biomolecular Techniques|date=July 2008|volume=19|issue=3|pages=151–158|pmid=19137101|pmc=2563918}}

External links

  • [https://web.archive.org/web/20090822064448/http://www.genome.ou.edu/protocol_book/protocol_index.html Protocols for Recombinant DNA Isolation, Cloning, and Sequencing]
{{Molecular biology}}

1 : Molecular biology
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