| 释义 |
- Function
- Model organisms
- References
- Further reading
{{Infobox_gene}}Nucleoporin 85 (Nup85) is a protein that in humans is encoded by the NUP85 gene.[1][2] Function Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160.[2] Model organisms Model organisms have been used in the study of NUP85 function. A conditional knockout mouse line called Nup85tm1a(KOMP)Wtsi was generated at the Wellcome Trust Sanger Institute.[3] Male and female animals underwent a standardized phenotypic screen[4] to determine the effects of deletion.[5][6][7][8] Additional screens performed: - In-depth immunological phenotyping[9] Nup85 knockout mouse phenotype| Characteristic | Phenotype |
|---|
| All data available at.[4][9] | Insulin | Normal | | Homozygous viability at P14 | Abnormal | | Recessive lethal study | Abnormal | | Body weight | Normal | | Neurological assessment | Normal | | Grip strength | Normal | | Dysmorphology | Normal | | Indirect calorimetry | Normal | | Glucose tolerance test | Normal | | Auditory brainstem response | Normal | | DEXA | Normal | | Radiography | Normal | | Eye morphology | Normal | | Clinical chemistry | Normal | | Haematology 16 Weeks | Normal | | Peripheral blood leukocytes 16 Weeks | Normal | | Heart weight | Normal | | Salmonella infection | Normal | | Cytotoxic T Cell Function | Normal | | Anti-nuclear Antibody Assay | Normal | | Epidermal Immune Composition | Normal | | Influenza Challenge | Normal | |
{{clear|left}} References 1. ^{{cite journal | vauthors = Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M | title = Pericentrin, a highly conserved centrosome protein involved in microtubule organization | journal = Cell | volume = 76 | issue = 4 | pages = 639–50 | date = Feb 1994 | pmid = 8124707 | pmc = | doi = 10.1016/0092-8674(94)90504-5 }}
2. ^1 {{cite web | title = Entrez Gene: NUP85 nucleoporin 85kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=79902| accessdate = }}
3. ^{{cite journal |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |journal=Acta Ophthalmologica|volume=88 |pages=925–7|doi=10.1111/j.1755-3768.2010.4142.x }}
4. ^1 {{cite web |url=http://www.mousephenotype.org/data/search?q=Nup85#fq=**&facet=gene |title=International Mouse Phenotyping Consortium}}
5. ^{{cite journal | vauthors = Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–42 | date = Jun 2011 | pmid = 21677750 | pmc = 3572410 | doi = 10.1038/nature10163 }}
6. ^{{cite journal | vauthors = Dolgin E | title = Mouse library set to be knockout | journal = Nature | volume = 474 | issue = 7351 | pages = 262–3 | date = Jun 2011 | pmid = 21677718 | doi = 10.1038/474262a }}
7. ^{{cite journal | vauthors = Collins FS, Rossant J, Wurst W | title = A mouse for all reasons | journal = Cell | volume = 128 | issue = 1 | pages = 9–13 | date = Jan 2007 | pmid = 17218247 | doi = 10.1016/j.cell.2006.12.018 }}
8. ^{{cite journal | vauthors = White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP | title = Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes | journal = Cell | volume = 154 | issue = 2 | pages = 452–64 | date = Jul 2013 | pmid = 23870131 | pmc = 3717207 | doi = 10.1016/j.cell.2013.06.022 }}
9. ^1 {{cite web |url= http://www.immunophenotyping.org/data/search?keys=Nup85&field_gene_construct_tid=All |title=Infection and Immunity Immunophenotyping (3i) Consortium}}
Further reading {{refbegin | 2}}- {{cite journal | vauthors = Kapeller R, Toker A, Cantley LC, Carpenter CL | title = Phosphoinositide 3-kinase binds constitutively to alpha/beta-tubulin and binds to gamma-tubulin in response to insulin | journal = The Journal of Biological Chemistry | volume = 270 | issue = 43 | pages = 25985–91 | date = Oct 1995 | pmid = 7592789 | doi = 10.1074/jbc.270.43.25985 }}
- {{cite journal | vauthors = Tynan SH, Purohit A, Doxsey SJ, Vallee RB | title = Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin | journal = The Journal of Biological Chemistry | volume = 275 | issue = 42 | pages = 32763–8 | date = Oct 2000 | pmid = 10893222 | doi = 10.1074/jbc.M001536200 }}
- {{cite journal | vauthors = Cronshaw JM, Krutchinsky AN, Zhang W, Chait BT, Matunis MJ | title = Proteomic analysis of the mammalian nuclear pore complex | journal = The Journal of Cell Biology | volume = 158 | issue = 5 | pages = 915–27 | date = Sep 2002 | pmid = 12196509 | pmc = 2173148 | doi = 10.1083/jcb.200206106 }}
- {{cite journal | vauthors = Mulari MT, Patrikainen L, Kaisto T, Metsikkö K, Salo JJ, Väänänen HK | title = The architecture of microtubular network and Golgi orientation in osteoclasts--major differences between avian and mammalian species | journal = Experimental Cell Research | volume = 285 | issue = 2 | pages = 221–35 | date = May 2003 | pmid = 12706117 | doi = 10.1016/S0014-4827(03)00033-8 }}
- {{cite journal | vauthors = Chang F, Re F, Sebastian S, Sazer S, Luban J | title = HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes | journal = Molecular Biology of the Cell | volume = 15 | issue = 4 | pages = 1793–801 | date = Apr 2004 | pmid = 14767062 | pmc = 379276 | doi = 10.1091/mbc.E03-09-0691 }}
- {{cite journal | vauthors = Loïodice I, Alves A, Rabut G, Van Overbeek M, Ellenberg J, Sibarita JB, Doye V | title = The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis | journal = Molecular Biology of the Cell | volume = 15 | issue = 7 | pages = 3333–44 | date = Jul 2004 | pmid = 15146057 | pmc = 452587 | doi = 10.1091/mbc.E03-12-0878 }}
- {{cite journal | vauthors = Terashima Y, Onai N, Murai M, Enomoto M, Poonpiriya V, Hamada T, Motomura K, Suwa M, Ezaki T, Haga T, Kanegasaki S, Matsushima K | title = Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis | journal = Nature Immunology | volume = 6 | issue = 8 | pages = 827–35 | date = Aug 2005 | pmid = 15995708 | doi = 10.1038/ni1222 }}
- {{cite journal|authorlink30=Huda Zoghbi | vauthors = Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M | title = Towards a proteome-scale map of the human protein-protein interaction network | journal = Nature | volume = 437 | issue = 7062 | pages = 1173–8 | date = Oct 2005 | pmid = 16189514 | doi = 10.1038/nature04209 }}
- {{cite journal | vauthors = Satoh M, Akatsu T, Ishkawa Y, Minami Y, Nakamura M | title = A novel activator of C-C chemokine, FROUNT, is expressed with C-C chemokine receptor 2 and its ligand in failing human heart | journal = Journal of Cardiac Failure | volume = 13 | issue = 2 | pages = 114–9 | date = Mar 2007 | pmid = 17395051 | doi = 10.1016/j.cardfail.2006.11.003 }}
{{refend}}{{Autoantigens}}{{gene-17-stub}} 1 : Nuclear pore complex |