“ORC1”的意思、由来-开放百科全书

Function

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of the pre-replication complex, which includes additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the origin recognition complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MYST2/HBO1), a protein involved in control of transcription silencing.^[2]

References

1. ^{{cite journal | vauthors = Eki T, Okumura K, Amin A, Ishiai M, Abe M, Nogami M, Taguchi H, Hurwitz J, Murakami Y, Hanaoka F | title = Mapping of the human homologue (ORC1L) of the yeast origin recognition complex subunit 1 gene to chromosome band 1p32 | journal = Genomics | volume = 36 | issue = 3 | pages = 559–61 | date = Sep 1996 | pmid = 8884289 | pmc = | doi = 10.1006/geno.1996.0511 }}
2. ^¹{{cite web | title = Entrez Gene: ORC1L origin recognition complex, subunit 1-like (yeast)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4998| accessdate = }}
3. ^{{cite journal | vauthors = Saha P, Chen J, Thome KC, Lawlis SJ, Hou ZH, Hendricks M, Parvin JD, Dutta A | title = Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase | journal = Molecular and Cellular Biology | volume = 18 | issue = 5 | pages = 2758–67 | date = May 1998 | pmid = 9566895 | pmc = 110655 | doi = 10.1128/mcb.18.5.2758}}
4. ^{{cite journal | vauthors = Iizuka M, Stillman B | title = Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein | journal = The Journal of Biological Chemistry | volume = 274 | issue = 33 | pages = 23027–34 | date = Aug 1999 | pmid = 10438470 | doi = 10.1074/jbc.274.33.23027}}
5. ^¹²³⁴⁵⁶⁷⁸⁹{{cite journal | vauthors = Kneissl M, Pütter V, Szalay AA, Grummt F | title = Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells | journal = Journal of Molecular Biology | volume = 327 | issue = 1 | pages = 111–28 | date = Mar 2003 | pmid = 12614612 | doi = 10.1016/s0022-2836(03)00079-2}}
6. ^{{cite journal | vauthors = Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T | title = Nuclear organization of DNA replication initiation proteins in mammalian cells | journal = The Journal of Biological Chemistry | volume = 277 | issue = 12 | pages = 10354–61 | date = Mar 2002 | pmid = 11779870 | doi = 10.1074/jbc.M111398200 }}
7. ^{{cite journal | vauthors = Vashee S, Simancek P, Challberg MD, Kelly TJ | title = Assembly of the human origin recognition complex | journal = The Journal of Biological Chemistry | volume = 276 | issue = 28 | pages = 26666–73 | date = Jul 2001 | pmid = 11323433 | doi = 10.1074/jbc.M102493200 }}
8. ^¹²{{cite journal | vauthors = Méndez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B | title = Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication | journal = Molecular Cell | volume = 9 | issue = 3 | pages = 481–91 | date = Mar 2002 | pmid = 11931757 | doi = 10.1016/s1097-2765(02)00467-7}}

Function

Interactions

References

Further reading