| 释义 |
- Function
- Model organisms
- References
- Further reading
{{Infobox_gene}}DNA-directed RNA polymerase III subunit RPC6 is an enzyme that in humans is encoded by the POLR3F gene.[1][2] Function The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.[2] Model organisms Model organisms have been used in the study of POLR3F function. A conditional knockout mouse line called Polr3ftm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute.[3] Male and female animals underwent a standardized phenotypic screen[4] to determine the effects of deletion.[5][6][7][8] Additional screens performed: - In-depth immunological phenotyping[9] Polr3f knockout mouse phenotype| Characteristic | Phenotype |
|---|
| All data available at.[4][9] | | Insulin | Normal | | Homozygous viability at P14 | Abnormal | | Recessive lethal study | Abnormal | | Body weight | Normal | | Neurological assessment | Normal | | Grip strength | Normal | | Dysmorphology | Normal | | Indirect calorimetry | Normal | | Glucose tolerance test | Normal | | Auditory brainstem response | Normal | | DEXA | Normal | | Radiography | Normal | | Eye morphology | Normal | | Clinical chemistry | Normal | | Haematology 16 Weeks | Normal | | Peripheral blood leukocytes 16 Weeks | Normal | | Micronucleus test | Abnormal | | Salmonella infection | Normal | | Epidermal Immune Composition | Normal | | Influenza Challenge | Normal | |
{{clear|left}} References 1. ^{{cite journal | vauthors = Wang Z, Roeder RG | title = Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation | journal = Genes & Development | volume = 11 | issue = 10 | pages = 1315–26 | date = May 1997 | pmid = 9171375 | pmc = | doi = 10.1101/gad.11.10.1315 }}
2. ^1 {{cite web | title = Entrez Gene: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10621| accessdate = }}
3. ^{{cite journal |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |journal=Acta Ophthalmologica|volume=88 |pages=925–7|doi=10.1111/j.1755-3768.2010.4142.x }}
4. ^1 {{cite web |url=http://www.mousephenotype.org/data/search?q=Polr3f#fq=**&facet=gene |title=International Mouse Phenotyping Consortium}}
5. ^{{cite journal | vauthors = Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–42 | date = Jun 2011 | pmid = 21677750 | pmc = 3572410 | doi = 10.1038/nature10163 }}
6. ^{{cite journal | vauthors = Dolgin E | title = Mouse library set to be knockout | journal = Nature | volume = 474 | issue = 7351 | pages = 262–3 | date = Jun 2011 | pmid = 21677718 | doi = 10.1038/474262a }}
7. ^{{cite journal | vauthors = Collins FS, Rossant J, Wurst W | title = A mouse for all reasons | journal = Cell | volume = 128 | issue = 1 | pages = 9–13 | date = Jan 2007 | pmid = 17218247 | doi = 10.1016/j.cell.2006.12.018 }}
8. ^{{cite journal | vauthors = White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP | title = Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes | journal = Cell | volume = 154 | issue = 2 | pages = 452–64 | date = Jul 2013 | pmid = 23870131 | pmc = 3717207 | doi = 10.1016/j.cell.2013.06.022 }}
9. ^1 {{cite web |url= http://www.immunophenotyping.org/data/search?keys=Polr3f&field_gene_construct_tid=All |title=Infection and Immunity Immunophenotyping (3i) Consortium}}
Further reading {{refbegin | 2}}- {{cite journal | vauthors = Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE | title = A human protein-protein interaction network: a resource for annotating the proteome | journal = Cell | volume = 122 | issue = 6 | pages = 957–68 | date = Sep 2005 | pmid = 16169070 | doi = 10.1016/j.cell.2005.08.029 }}
- {{cite journal | vauthors = Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP, Hernandez N | title = Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits | journal = Molecular and Cellular Biology | volume = 22 | issue = 22 | pages = 8044–55 | date = Nov 2002 | pmid = 12391170 | pmc = 134740 | doi = 10.1128/MCB.22.22.8044-8055.2002 | url = http://repository.cshl.edu/28722/1/Kobayashi%20Myers%20Hernandez%20Molecular%20and%20Cellular%20Biology%202002.pdf }}
- {{cite journal | vauthors = Jones E, Kimura H, Vigneron M, Wang Z, Roeder RG, Cook PR | title = Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III | journal = Experimental Cell Research | volume = 254 | issue = 1 | pages = 163–72 | date = Jan 2000 | pmid = 10623476 | doi = 10.1006/excr.1999.4739 }}
- {{cite journal | vauthors = Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG | title = The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity | journal = Molecular and Cellular Biology | volume = 19 | issue = 11 | pages = 7697–704 | date = Nov 1999 | pmid = 10523658 | pmc = 84812 | doi = 10.1128/mcb.19.11.7697}}
- {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1-2 | pages = 149–56 | date = Oct 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
- {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1-2 | pages = 171–4 | date = Jan 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
- {{cite journal | vauthors = Jang KL, Collins MK, Latchman DS | title = The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC | journal = Journal of Acquired Immune Deficiency Syndromes | volume = 5 | issue = 11 | pages = 1142–7 | year = 1992 | pmid = 1403646 | doi = }}
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