“RNA immunoprecipitation chip”的意思、由来-开放百科全书

A RNA immunoprecipitation chip (RIP-Chip) is immunoprecipitation of an RNA-binding protein coupled to reverse transcription and a microarray. It has been used to find interactions between RNA and protein (one protein but many RNA species per analysis).^[1]^[2]^[3]

RIP-Seq

An alternative methodology (RIP-Seq) is to sequence the RNAs that were pulled down using high-throughput sequencing rather than analyze them with a microarray.{{cn|date=February 2018}}

ChIP-on-chip

A similar technique is ChIP-on-chip, which detects the binding of proteins to genomic DNA rather than RNA.{{cn|date=February 2018}}

CLIP-Seq

A competing technique is CLIP-Seq, where the RNA binding protein is cross-linked to the RNA via the use of UV light, followed by nuclease digestion and analyzed with high-throughput sequencing.{{cn|date=February 2018}}

References

1. ^{{cite journal |vauthors=Townley-Tilson WH, Pendergrass SA, Marzluff WF, Whitfield ML |title=Genome-wide analysis of mRNAs bound to the histone stem–loop binding protein |journal=RNA |volume=12 |issue=10 |pages=1853–67 |date=October 2006 |pmid=16931877 |pmc=1581977 |doi=10.1261/rna.76006}}
2. ^{{cite journal |vauthors=Khalil AM, Guttman M, Huarte M, etal |title=Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression |journal=Proc. Natl. Acad. Sci. U.S.A. |date=July 2009 |pmid=19571010 |pmc=2704857 |doi=10.1073/pnas.0904715106 |volume=106 |issue=28 |pages=11667–72|url=http://dspace.mit.edu/bitstream/1721.1/52550/1/Khalil-2009-Many%20human%20large%20int.pdf }}
3. ^{{cite journal |vauthors= Hendrickson DG, Hogan DH, Herschlag D, ((Ferrell JEF)), Brown PB| title=Systematic Identification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance|journal=PLoS ONE| year = 2008| pmid=18461144 | doi = 10.1371/journal.pone.0002126| pmc=2330160 |editor1-last= Bähler |editor1-first= Jürg |volume= 3 |issue= 5 |pages= e2126 }}