“RpoS mRNA 5'UTR”的意思、由来-开放百科全书

rpoS mRNA encodes for the rpoS stress factor, sigma S (σ^S or σ³⁸) in Escherichia coli and related bacteria, where DsrA in conjunction with the Sm like RNA binding protein, Hfq promote the translation of this rpoS mRNA.^[1] The 5' UTR of the rpoS mRNA forms a self-inhibitory stem loop that shields the shine-dalgarno sequence and therefore inhibits translation. The secondary structure of the 5'UTR was predicted by acetylation of ribose 2'-hydroxyls with NMIA and by using a secondary structure prediction program, RNAstructure.^[2]^[3] DsrA stimulates rpoS translation by binding in the 5'UTR and causes the stem loop to open, exposing the ribosome binding site.

Site-directed mutagenesis and deletion analysis located an AAYAA motif identified upstream of the stem loop structure which is required for Hfq binding.^[4] Comparative genomics has shown that this regulatory secondary structure is conserved across several gram-negative bacteria species.

References

1. ^{{Cite journal| pmid = 12208995| date=Sep 2002 | last1 = Hengge-Aronis | first1 = R.| title = Signal Transduction and Regulatory Mechanisms Involved in Control of the σS (RpoS) Subunit of RNA Polymerase| volume = 66| issue = 3| pages = 373–395, table 395 contents| issn = 1092-2172| pmc = 120795| journal = Microbiology and Molecular Biology Reviews| doi = 10.1128/MMBR.66.3.373-395.2002}}
2. ^{{Cite journal| last3 = Coughlan | first1 = J. | first2 = A.| last2 = Wilkinson| first3 = L. | first4 = M. | title = RNA Structure Analysis at Single Nucleotide Resolution by Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE)| last1 = Merino | journal = Journal of the American Chemical Society| last4 = Weeks| volume = 127| issue = 12 | pages = 4223–4231 | date=Mar 2005 | issn = 0002-7863| pmid = 15783204 | doi = 10.1021/ja043822v}}
3. ^{{Cite journal| first1 = H.| last2 = Disney| last3 = Childs| last5 = Zuker| last4 = Schroeder | first2 = D. | first3 = L. | first4 = J. | first5 = M.| title = Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure| last1 = Mathews | first6 = H.| last6 = Turner| journal = Proceedings of the National Academy of Sciences | volume = 101| issue = 19 | pages = 7287–7292 | date=May 2004 | issn = 0027-8424| pmid = 15123812| pmc = 409911 | doi = 10.1073/pnas.0401799101|bibcode = 2004PNAS..101.7287M }}
4. ^{{Cite journal| first1 = J. | first2 = A. | title = The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA| last2 = Woodson| journal = RNA| last1 = Soper | volume = 14| issue = 9 | pages = 1907–1917 | date=Sep 2008 | issn = 1355-8382| pmid = 18658123| pmc = 2525945 | doi = 10.1261/rna.1110608}}