词条 | Rsa RNA |
释义 |
RsaARepresses the translation of the transcriptional regulator MgrA by binding to its mRNA, enhances biofilm formation and decreases bacterial virulence.[4] Other mRNAs: including SsaA-like enzymes involved in peptidoglycan metabolism and the secreted anti-inflammatory FLIPr protein were validated as direct targets of RsaA.[5] RsaERsaE is found in other members of the genus Staphylococcus such as Staphylococcus epidermidis and Staphylococcus saprophyticus and is the only Rsa RNA to be found outside of this genus, in Macrococcus caseolyticus and Bacillus. In Bacillus subtilis, RsaE had previously been identified as ncr22.[8][9] RsaE is also consistently found downstream of PepF which codes for oligoendopeptidase F. The function of RsaE was discovered using gene knockout analysis and gene overexpression - it was found to regulate the expression of several enzymes involved in metabolism via antisense binding of their mRNA.[1][3] RsaE was shown to be regulated by the presence of nitric oxide (NO). In Bacillus subtilis it controls expression of genes with functions related to oxidative stress and oxidation-reduction reactions and it was renamed RoxS (for related to oxidative stress).[10] RsaFIn S.aureus species RsaF is located in the same intergenic region as RsaE and overlaps with 3' end of RsaE by approximately 20bp. In contrast to RsaE, RsaF and its upstream gene have only been identified in S.aureus species.[1] RsaKRsaK is found in the leader sequence of glcA mRNA which encodes an enzyme involved in the glucose-specific phosphotransferase system. RsaK also contains a conserved ribonucleic antiterminator system, as recognised by GclT protein.[11] RsaIRsaOG[2] also renamed RsaI[1] is thought to fine-tune the regulation of toxin or invasion mechanisms in S. aureus via trans-acting mechanisms. Its secondary structure contains a pseudoknot formed between two highly conserved unpaired sequences.[6]Expression patternsRsaD, E H and I were found to be highly expressed in S. aureus. Expression levels of other Rsa RNAs varied under various environmental conditions, for example RsaC was induced by cold shock and RsaA is induced in response to osmotic stress.[1][2][3] RsaE and RsaF genes overlap in S.aureus species but appear to have opposite expression patterns.[1] Transcriptional interference due to an overlap between a σA recognition motif and a potential σB binding site is proposed as a mechanism causing the differential expression of the two transcripts[1][12] See also
References1. ^1 2 3 4 5 6 7 {{cite journal | vauthors = Geissmann T, Chevalier C, Cros MJ, Boisset S, Fechter P, Noirot C, Schrenzel J, François P, Vandenesch F, Gaspin C, Romby P | title = A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation | journal = Nucleic Acids Research | volume = 37 | issue = 21 | pages = 7239–57 | date = November 2009 | pmid = 19786493 | pmc = 2790875 | doi = 10.1093/nar/gkp668 | url = http://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=19786493 }} 2. ^1 2 {{cite journal | vauthors = Marchais A, Naville M, Bohn C, Bouloc P, Gautheret D | title = Single-pass classification of all noncoding sequences in a bacterial genome using phylogenetic profiles | journal = Genome Research | volume = 19 | issue = 6 | pages = 1084–92 | date = June 2009 | pmid = 19237465 | pmc = 2694484 | doi = 10.1101/gr.089714.108 }} 3. ^1 2 {{cite journal | vauthors = Bohn C, Rigoulay C, Chabelskaya S, Sharma CM, Marchais A, Skorski P, Borezée-Durant E, Barbet R, Jacquet E, Jacq A, Gautheret D, Felden B, Vogel J, Bouloc P | title = Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism | journal = Nucleic Acids Research | volume = 38 | issue = 19 | pages = 6620–36 | date = October 2010 | pmid = 20511587 | pmc = 2965222 | doi = 10.1093/nar/gkq462 | url = http://nar.oxfordjournals.org/cgi/content/full/gkq462v1?view=long&pmid=20511587 }} 4. ^{{cite journal | vauthors = Romilly C, Lays C, Tomasini A, Caldelari I, Benito Y, Hammann P, Geissmann T, Boisset S, Romby P, Vandenesch F | title = A non-coding RNA promotes bacterial persistence and decreases virulence by regulating a regulator in Staphylococcus aureus | journal = PLoS Pathogens | volume = 10 | issue = 3 | pages = e1003979 | date = March 2014 | pmid = 24651379 | pmc = 3961350 | doi = 10.1371/journal.ppat.1003979 }} 5. ^{{cite journal | vauthors = Tomasini A, Moreau K, Chicher J, Geissmann T, Vandenesch F, Romby P, Marzi S, Caldelari I | title = The RNA targetome of Staphylococcus aureus non-coding RNA RsaA: impact on cell surface properties and defense mechanisms | journal = Nucleic Acids Research | volume = 45 | issue = 11 | pages = 6746–6760 | date = June 2017 | pmid = 28379505 | pmc = 5499838 | doi = 10.1093/nar/gkx219 }} 6. ^1 {{cite journal | vauthors = Marchais A, Bohn C, Bouloc P, Gautheret D | title = RsaOG, a new staphylococcal family of highly transcribed non-coding RNA | journal = RNA Biology | volume = 7 | issue = 2 | pages = 116–9 | date = March 2010 | pmid = 20200491 | doi = 10.4161/rna.7.2.10925 | url = http://www.landesbioscience.com/journals/rna/abstract.php?id=10925 }} 7. ^{{cite journal | vauthors = Darty K, Denise A, Ponty Y | title = VARNA: Interactive drawing and editing of the RNA secondary structure | journal = Bioinformatics | volume = 25 | issue = 15 | pages = 1974–5 | date = August 2009 | pmid = 19398448 | pmc = 2712331 | doi = 10.1093/bioinformatics/btp250 | url = http://bioinformatics.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=19398448 }} 8. ^{{cite journal | vauthors = Rasmussen S, Nielsen HB, Jarmer H | title = The transcriptionally active regions in the genome of Bacillus subtilis | journal = Molecular Microbiology | volume = 73 | issue = 6 | pages = 1043–57 | date = September 2009 | pmid = 19682248 | pmc = 2784878 | doi = 10.1111/j.1365-2958.2009.06830.x }} 9. ^{{cite journal | vauthors = Irnov I, Sharma CM, Vogel J, Winkler WC | title = Identification of regulatory RNAs in Bacillus subtilis | journal = Nucleic Acids Research | volume = 38 | issue = 19 | pages = 6637–51 | date = October 2010 | pmid = 20525796 | pmc = 2965217 | doi = 10.1093/nar/gkq454 | url = http://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=20525796 }} 10. ^{{cite journal | vauthors = Durand S, Braun F, Lioliou E, Romilly C, Helfer AC, Kuhn L, Quittot N, Nicolas P, Romby P, Condon C | title = A nitric oxide regulated small RNA controls expression of genes involved in redox homeostasis in Bacillus subtilis | journal = PLoS Genetics | volume = 11 | issue = 2 | pages = e1004957 | date = February 2015 | pmid = 25643072 | pmc = 4409812 | doi = 10.1371/journal.pgen.1004957 }} 11. ^{{cite journal | vauthors = Langbein I, Bachem S, Stülke J | title = Specific interaction of the RNA-binding domain of the bacillus subtilis transcriptional antiterminator GlcT with its RNA target, RAT | journal = Journal of Molecular Biology | volume = 293 | issue = 4 | pages = 795–805 | date = November 1999 | pmid = 10543968 | doi = 10.1006/jmbi.1999.3176 | url = http://linkinghub.elsevier.com/retrieve/pii/S0022-2836(99)93176-5 }} 12. ^{{cite journal | vauthors = Shearwin KE, Callen BP, Egan JB | title = Transcriptional interference--a crash course | journal = Trends in Genetics | volume = 21 | issue = 6 | pages = 339–45 | date = June 2005 | pmid = 15922833 | pmc = 2941638 | doi = 10.1016/j.tig.2005.04.009 }} Further reading{{refbegin|32em}}
| name = hidden | title = Gallery of Rsa RNA secondary structure images | titlestyle = background:#e7dcc3 | state = autocollapse | list1 = {{Gallery | lines=3 | Image:RsaA SScons.png|RsaA: Secondary structure of RsaA. Rfam family RF01816 | Image:RsaB SScons.png|RsaB: Secondary structure of RsaB. Rfam family RF01817 | Image:RsaC SScons.png|RsaC: Secondary structure of RsaC. Rfam family RF01818 | Image:RsaD SScons.png|RsaD: Secondary structure of RsaD. Rfam family RF01819 | Image:RsaE SScons.png|RsaE: Secondary structure of RsaE. Rfam family RF01820 | Image:RsaF SScons.png|RsaF: Secondary structure of RsaF. Rfam family RF01858 | Image:RsaG SScons.png|RsaG: Secondary structure of RsaG. | Image:RsaH SScons.png|RsaH: Secondary structure of RsaH. Rfam family RF01821 | Image:RsaI SScons.png|RsaI: Secondary structure of RsaI. Rfam family RF01775 | Image:RsaJ SScons.png|RsaJ: Secondary structure of RsaJ. Rfam family RF01822 |}} }} 1 : Non-coding RNA |
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