“SENP1”的意思、由来-开放百科全书

General

So far there are six SUMO proteases in humans that have been designated SENP1-3 and SENP5-7 (sentrin/SUMO-specific protease).1 The six proteases possess a conserved C-terminal domain which are variable in size, and with a distinct N-terminal domain between them. The C-terminal domain shows catalytic activity and N-terminal domain regulates cell localization and substrate specificity.^[4]

Features

SENP1 (Sentrin-specific protease 1) is a human protease of 643 amino acids with a weight of 73 kDa, EC number in humans 3.4.22.B70, which adopts a conformation that identifies it as a member of the superfamily of cysteine proteases contain a catalytic triad with characterized three amino acids: a cysteine at position 602, a histidine at position 533 and aspartic acid at position 550.

The important nucleophile is cysteine located at the N-terminal alpha helix of the protein core, the other two amino acids, aspartate and histidine, are located in a beta sheet end.

Location

Both SENP1 are located in the nucleus and cytosol depending on the cell type, although it has been seen that is exported out from the nucleus to the cytosol through a sequence of nuclear export (NES) that is located at the C-terminus. The mammalian SENP1 is localized mainly in the nucleus.^[6]

Function

SENP1 catalyzes maturation SUMO protein (small ubiquitin-related modifier), which causes hydrolysis peptide bond of SUMO is in a conserved sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal ^[7] to be added to the conjugation of other proteins (sumoylation).^[8]

In vertebrates there are three members of the family of SUMO: SUMO-1, -2 and -3. SENP1 can catalyze any of these three.

This conjugation of SUMO toward other proteins is a lot like ubiquitination, however these modifications leads to different results depending on the type of protein been modified.^[9]

References

1. ^{{cite journal | vauthors = Gong L, Millas S, Maul GG, Yeh ET | title = Differential regulation of sentrinized proteins by a novel sentrin-specific protease | journal = The Journal of Biological Chemistry | volume = 275 | issue = 5 | pages = 3355–9 | date = Feb 2000 | pmid = 10652325 | pmc = | doi = 10.1074/jbc.275.5.3355 }}
2. ^{{cite journal | vauthors = Bailey D, O'Hare P | title = Characterization of the localization and proteolytic activity of the SUMO-specific protease, SENP1 | journal = The Journal of Biological Chemistry | volume = 279 | issue = 1 | pages = 692–703 | date = Jan 2004 | pmid = 14563852 | pmc = | doi = 10.1074/jbc.M306195200 }}
3. ^{{cite web | title = Entrez Gene: SENP1 SUMO1/sentrin specific peptidase 1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=29843| accessdate = }}
4. ^{{cite journal | vauthors = Xu Z, Chau SF, Lam KH, Chan HY, Ng TB, Au SW | title = Crystal structure of the SENP1 mutant C603S-SUMO complex reveals the hydrolytic mechanism of SUMO-specific protease | journal = The Biochemical Journal | volume = 398 | issue = 3 | date = Sep 2006 | pmid = 16712526 | doi = 10.1042/BJ20060526 | pmc=1559472 | pages=345–52}}
5. ^{{cite journal | vauthors = Shen LN, Dong C, Liu H, Naismith JH, Hay RT | year = 2006 | title = The structure of SENP1-SUMO-2 complex suggests a structural basis for discrimination between SUMO paralogues during processing | url = | journal = The Biochemical Journal | volume = 397 | issue = 2| pages = 279–288 | doi = 10.1042/BJ20052030 | pmid=16553580 | pmc=1513277}}
6. ^{{cite journal | vauthors = Kim YH, Sung KS, Lee SJ, Kim YO, Choi CY, Kim Y | year = 2005 | title = Desumoylation of homeodomain-interacting protein kinase 2 (HIPK2) through the cytoplasmic-nuclear shuttling of the SUMO-specific protease SENP1 | url = | journal = FEBS Letters | volume = 579 | issue = 27| pages = 6272–6278 | doi = 10.1016/j.febslet.2005.10.010 | pmid=16253240}}
7. ^{{Cite web | url=https://www.uniprot.org/uniprot/Q9P0U3#ptm_processing | title=SENP1 - Sentrin-specific protease 1 - Homo sapiens (Human) - SENP1 gene & protein}}
8. ^{{cite journal | vauthors = Xu Z, Au SW | year = 2005 | title = Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1 | url = | journal = The Biochemical Journal | volume = 386 | issue = Pt 2| pages = 325–330 | doi = 10.1042/BJ20041210 | pmid=15487983 | pmc=1134797}}
9. ^{{cite journal | vauthors = Xu Z, Chau SF, Lam KH, Chan HY, Ng TB, Au SW | title = Crystal structure of the SENP1 mutant C603S-SUMO complex reveals the hydrolytic mechanism of SUMO-specific protease | journal = The Biochemical Journal | volume = 398 | issue = 3 | date = 2006 | pmid = 16712526 | doi = 10.1042/BJ20060526 | pmc=1559472 | pages=345–52}}

General

Features

Location

Function

References

Further reading