MDO may refer to:

• MDO (band), a Puerto Rican boy band, a spin-off from Menudo
• Marine Diesel Oil, a type of fuel oil used in the maritime field
• Medium density overlay, a type of plywood
• Multidisciplinary design optimization, a field of engineering
• Mixed-domain oscilloscope, a kind of oscilloscope that allows combining information from time and frequency domain
• MOD - Markup Declaration Open: "{{Disambiguation}}

• Chesapeake, Virginia, USA
• Chesapeake Warehouses
• Chesapeake–Leopard Incident
• Che sara
• Che sarà
• Chesau
• Chesay
• Chesb
• Chesbro Dam
• Chesbro Reservoir County Park
• Chesca Miles
• Chesebro
• Chesebro Smith House
• Chesebrough-Pond's
• Chesebrough-Pond's, Inc.
• Cheselden
• Chesenhale
• Chesepian
• Chesepiooc
• Che-se-quah
• Chesereu
• Cheser Mog
• Chesewell
• Chesgin
• Chesha Bay