Step off may refer to:

• Step-off, a parameter in estimating the severity of injury of the posterior cruciate ligament
• Step Off (album)
• Step Off, 1984 Melle Mel song

• South Island adzebill
• South Island brown kiwi
• South Island kokako
• South Island lichen moth
• South Island Line
• South Island Line (West)
• South Island Robin
• South Jamaica houses
• South Jamesport, New York
• South Japan
• South Jefferson Avenue Historic District
• South Jersey Port Corporation
• South Jew Hist
• South. Jew. Hist.
• South Jordan Parkway (UTA station)
• South Jordan (UTA station)
• South J Phil
• South. J. Phil.
• South. J. Philos.
• South J Philos
• South Junior High School (Quincy, Massachusetts)
• South Kalapuya
• South Kamberau language
• South Kamrup district
• South kamrup district