Thomas Gillespie may refer to:

• Thomas Gillespie (minister) (1708–1774), Scottish church leader
• Thomas Gillespie (Wisconsin politician) (1831–1914)
• Thomas Gillespie (rower) (1892–1914)
• Thomas Andrew Gillespie (1852–1926), American businessman known for T. A. Gillespie Company Shell Loading Plant explosion
• Thomas F. Gillespie (1838–1893), Irish-born merchant and political figure in Canada
• Thomas Haining Gillespie (1876–1967), Scottish zoologist and broadcaster

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