| 词条 | Trinder glucose activity test |
| 释义 |
| name = Trinder glucose activity test | image = | alt = | caption = | pronounce = | synonyms = |purpose = determine the presence of glucose | reference_range = | calculator = | DiseasesDB = | ICD10 = | ICD9 = | ICDO = | MedlinePlus = | eMedicine = | MeshID = | OPS301 = | LOINC = }} The Trinder glucose activity test is a diagnostic test used in medicine to determine the presence of glucose or glucose oxidase. The test employs the Trinder reagent, and is a colour change test resulting from the Trinder reaction. The Trinder reagent, named after P. Trinder of the Biochemistry Department of the Royal Infirmary in Sunderland (see the article listed in further reading), comprises an aminoantipyrine (such as 4-aminoantipyrine) and phenol (p-hydroxybenzene).[1][2] The Trinder reaction is the reaction between hydrogen peroxide and the phenol and aminoantipyrine to form a quinone (quinoneimine), catalyzed by the presence of a peroxidase (such as horseradish peroxidase).[1][2][3][4][5] The hydrogen peroxide is itself produced by an initial reaction where the glucose is oxidised in the presence of the glucose oxidase catalyst into H2O2 and gluconic acid.[2][3] The quinone is red-violet in colour,[3][5] with the intensity of the colour being in proportion to the glucose concentration.[3] The colour is measured at 505 nm,[2] 510 nm,[4] or 540 nm.[3] Diagnostic kits containing the Trinder reagent are available, including one from Sigma-Aldrich.[2] The Stanbio Single Reagent Glucose Method is based upon the Trinder technique.[3][6] References1. ^1 {{cite book|title=Nonisotopic immunoassay|author=That Tjien Ngo|page=71|publisher=Plenum Press|year=1988}} 2. ^1 2 3 4 {{cite conference|booktitle=Chemical and biological sensors and analytical methods II: proceedings of the international symposium|volume=2001-18|editor1=Michael Alan Butler |editor2=P. Vanýsek |editor3=Noboru Yamazoe |publisher=The Electrochemical Society|year=2001|isbn=9781566773515|title=Biosensors From Conductive Polymer Transducers and Sol-Gel Encapsulated Bioindicator Molecules|author1=F. Yamagishi |author2=T. Stanford |author3=C. van Ast |pages=223}} 3. ^1 2 3 4 5 {{cite book|title=Industrial Pollution & Management|editor=Arvind Kumar|publisher=APH Publishing|year=2004|isbn=9788176487740|author=Arvind Kumar, Rajiv Kr. Mishra, & Sudhanshu S. Roy|chapter=Studies on Impact of Industrial Pollution on Biochemical and Histological Changes in a Catfish, Mystus vittatus (Bloch)|page=9}} 4. ^1 {{cite book|title=Enzyme technology|editor1=Ashok Pandey |editor2=Colin Webb |editor3=Carlos Ricardo Soccol |editor4=Christian Larroche |page=410|publisher=Springer|year=2006|isbn=9780387292946 |chapter=Peroxidases|author1=Carlos G. Dosoretz |author2=Gary Ward }} 5. ^1 {{cite book|title=Autotrophic microbiology and one-carbon metabolism|editor1=Geoffrey A. Codd |editor2=Lubbert Dijkhuizen |editor3=F. Robert Tabita |page=219|publisher=Kluwer Academic Publishers|isbn=978-0-7923-0656-6|year=1990|chapter=Biochemistry and Applications of Alcohol Oxidase from Methylotrophic Yeasts|author=J.R. Woodward}} 6. ^{{cite journal|title=Effects of rumen fill on short-term ingestive behavior and circulating concentrations of ghrelin, insulin, and glucose of dairy cows foraging vegetative micro-swards|author1=P. Gregorini |author2=K. J. Soder |author3=R. S. Kensinger |journal=J. Dairy Sci.|year=2009|volume=92|pages=2095–2105|doi=10.3168/jds.2008-1803|pmid=19389967|issue=5|url=https://naldc-legacy.nal.usda.gov/naldc/download.xhtml?id=30371&content=PDF}} Further reading
1 : Medical tests |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。