| 词条 | Viability assay |
| 释义 |
A viability assay is an assay to determine the ability of organs, cells or tissues to maintain or recover viability. Viability can be distinguished from the all-or-nothing states of life and death by use of a quantifiable index between 0 and 1 (or 0% and 100%).[1] Viability can assay mechanical activity, motility (spermatozoa or granulocytes), contraction (muscle tissue or cells), mitotic activity, etc.[1] For example, examining the ratio of potassium to sodium in cells can serve as an index of viability. If the cells do not have high intracellular potassium and low intracellular sodium, then (1) the cell membrane may not be intact, and/or (2) the sodium-potassium pump may not be operating well [2][3] As with many kinds of viability assays, quantitative measures of physiological function do not indicate whether damage repair and recovery is possible.[4] An assay of the ability of a cell line to adhere and divide may be more indicative of incipient damage than membrane integrity.[5] Fluorescent-based assays do not require large sample sizes.[6] Viability assays are used to assess the success of cryopreservation techniques, the toxicity of substances, or the effectiveness of substances in mitigating effects of toxic substances. Classification
List of common viability assays
See also
References1. ^1 {{cite journal | author=Pegg DE | title=Viability assays for preserved cells, tissues, and organs | journal=Cryobiology | volume=26 | issue=3 | year=1989 | pages=212–231 | id= | pmid=2743785 | doi=10.1016/0011-2240(89)90016-3}} 2. ^{{cite journal |vauthors=Lindner B, Seydel U | title=Mass spectrometric analysis of drug-induced changes in Na+ and K+ contents of single bacterial cells | journal=Journal of General Microbiology | volume=129 | issue=1 | year=1983 | pages=51–55 | url = http://mic.sgmjournals.org/cgi/reprint/129/1/51 | id= | pmid=6339677 | doi=10.1099/00221287-129-1-51}} 3. ^{{cite journal |vauthors=Pichugin Y, Fahy GM, Morin R | title=Cryopreservation of rat hippocampal slices by vitrification | journal=Cryobiology | volume=52 | issue=2 | year=2006 | pages=228–240 | url = http://www.21cm.com/pdfs/hippo_published.pdf | id= | pmid=16403489 | doi=10.1016/j.cryobiol.2005.11.006}} 4. ^{{cite journal |vauthors=Crutchfield A, Diller K, Brand J | title=Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta) | journal=European Journal of Phycology | volume=34 | issue=1 | year=1999 | pages=43–52 | url = http://www.ingentaconnect.com/content/tandf/tejp/1999/00000034/00000001/art00006| doi = 10.1080/09670269910001736072 }} 5. ^{{cite journal |vauthors=Wusteman MC, Pegg DE, Robinson MP, Wang LH, Fitch P | title=Vitrification media: toxicity, permeability, and dielectric properties | journal=Cryobiology | volume=44 | issue=1 | year=2002 | pages=24–37 | id= | pmid=12061845 | doi=10.1016/S0011-2240(02)00002-0}} 6. ^{{cite web | title = Overview of Probes for Cell Viability, Cell Proliferation and Live-Cell Function—Section 15.1 | work = Invitrogen | publisher = Life Technologies | year = 2010 | url = http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Assays-for-Cell-Viability-Proliferation-and-Function/Overview-of-Probes-for-Cell-Viability-Cell-Proliferation-and-Live-Cell-Function.html | accessdate = 2010-10-15 }} 7. ^{{cite journal |vauthors=Henkelman S, Lagerberg JW, Graaff R, Rakhorst G, Van Oeveren W | title=The effects of cryopreservation on red blood cell rheologic properties | journal=Transfusion | volume=50 | issue=11 | year=2010 | pages=2393–2401 | doi = 10.1111/j.1537-2995.2010.02730.x| id= | pmid=20561300}} 8. ^{{cite journal | author=Southard JH | title=Viability assays in organ preservation | journal=Cryobiology | volume=26 | issue=3 | year=1989 | pages=232–238 | id= | pmid=2663353 | doi=10.1016/0011-2240(89)90017-5}} 9. ^{{cite journal |author=Lecoeur H |title=Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases |journal=Experimental Cell Research|volume=277 |issue=1 |pages=1–14 |year=2002 |pmid=12061813 |doi=10.1006/excr.2002.5537}} Further reading
4 : Cell biology|Molecular biology techniques|Microbiology techniques|Toxicology tests |
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