Zamenhof is a Polish-Jewish surname. Notable people with the surname include:

• L. L. Zamenhof (1859–1917), Polish ophthalmologist, philologist, and the inventor of Esperanto
• Adam Zamenhof (1888–1940), Polish ophthalmologist, the son of L. L. Zamenhof
• Lidia Zamenhof (1904–1942), Polish Esperantist, the daughter of L. L. Zamenhof
• Louis-Christophe Zaleski-Zamenhof (1925–), civil and marine engineer, grandson of L. L. Zamenhof

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