Chamberlayne is a surname. Notable people with the surname include:

• Edward Chamberlayne (1616–1703), English writer
• Tankerville Chamberlayne (1840–1924), English Member of Parliament
• Thomas Chamberlayne (disambiguation) several men with this name
• William Chamberlayne (poet) (1619–1679), English poet
• William Chamberlayne (MP) (1760–1829), English Member of Parliament and landowner
• The Chamberlayne family, an influential family of Weston, Southampton, United Kingdom

• Pudhumai Penn
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