“Crab duplex-specific nuclease”的意思、由来-开放百科全书

词条

Crab duplex-specific nuclease

释义

References

{{Orphan|date=April 2011}}Crab duplex-specific nuclease is a nuclease derived from kamchatka crab hepatopancreas that displays a strong preference for cleaving double-stranded DNA and DNA in DNA–RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for non-perfectly matched duplexes of the same length. It has been applied to SNP detection^[1] and RNA normalization.^[2]

References

1. ^{{cite journal|last=Shagin|first=DA |author2=Rebrikov, DV |author3=Kozhemyako, VB |author4=Altshuler, IM |author5=Shcheglov, AS |author6=Zhulidov, PA |author7=Bogdanova, EA |author8=Staroverov, DB |author9=Rasskazov, VA |author10=Lukyanov, S|title=A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas|journal=Genome Research|date=December 2002|volume=12|issue=12|pages=1935–42|pmid=12466298|doi=10.1101/gr.547002|pmc=187582}}
2. ^{{cite journal|last=Christodoulou|first=DC |author2=Gorham, JM |author3=Herman, DS |author4=Seidman, JG|title=Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease |journal=Current Protocols in Molecular Biology|date=April 2011|volume=Chapter 4|pages=Unit 4.12|pmid=21472699|doi=10.1002/0471142727.mb0412s94|pmc=3152986}}

2 : King crabs|EC 3.1

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