There were 16 female and 41 male athletes representing the country at the 2000 Summer Paralympics.[1]
| Medal | Name | Sport | Event |
|---|
| {{gold medal}} | Zdenek Sebek | Archery | Men's individual W1 |
| {{gold medal}} | Milan Kubala | Athletics | Men's discus F36 |
| {{gold medal}} | Martin Němec | Athletics | Men's discus F55 |
| {{gold medal}} | Roman Musil | Athletics | Men's javelin F33 |
| {{gold medal}} | Roman Musil | Athletics | Men's shot put F33 |
| {{gold medal}} | Martina Kniezkova | Athletics | Women's discus F51-54 |
| {{gold medal}} | Roman Musil | Cycling | Mixed tricycle 1.9km time trial CP div 2 |
| {{gold medal}} | Jiri Jezek | Cycling | Mixed 1km time trial LC2 |
| {{gold medal}} | Jiri Jezek | Cycling | Mixed individual pursuit LC2 |
| {{gold medal}} | Martin Kovar | Swimming | Men's 50m backstroke S3 |
| {{gold medal}} | Ivana Kumpostova | Swimming | Women's 50m breaststroke SB14 |
| {{gold medal}} | Jana Hoffmanova | Swimming | Women's 50m freestyle S3 |
| {{gold medal}} | Michal Stefanu | Table tennis | Men's singles 4 |
| {{gold medal}} | Ivan Karabec | Table tennis | Men's singles 10 |
| {{gold medal}} | Jolana Davidkova | Table tennis | Women's singles 10 |
| {{silver medal}} | Petr Novak | Athletics | Men's 100m T11 |
| {{silver medal}} | Roman Musil | Athletics | Men's discus F33 |
| {{silver medal}} | Roman Kolek | Athletics | Men's discus F38 |
| {{silver medal}} | Frantisek Purgl | Athletics | Men's discus F54 |
| {{silver medal}} | Josef Stiak | Athletics | Men's javelin F56 |
| {{silver medal}} | Rostislav Pohlmann | Athletics | Men's javelin F57 |
| {{silver medal}} | Martin Němec | Athletics | Men's shot put F55 |
| {{silver medal}} | Vladimira Bujarkova | Athletics | Women's discus F37 |
| {{silver medal}} | Martina Kniezkova | Athletics | Women's javelin F52-54 |
| {{silver medal}} | Jiri Jezek | Cycling | Mixed bicycle road race LC2 |
| {{silver medal}} | Katerina Liskova | Swimming | Women's 50m backstroke S5 |
| {{silver medal}} | Ivana Kumpostova | Swimming | Women's 50m backstroke S14 |
| {{silver medal}} | Tomas Pribyl
Martin Zvolanek | Table tennis | Men's teams 1-2 |
| {{silver medal}} | Lubomir Masek
Miroslav Cinibulk
Pavel Cech | Table tennis | Men's team 10 |
| {{silver medal}} | Jolana Davidkova
Jana Mojova
Eva Pestova
Michala Zakova | Table tennis | Women's teams 6-10 |
| {{bronze medal}} | Ales Svehlik | Athletics | Men's 5000m T38 |
| {{bronze medal}} | Roman Musil | Cycling | Mixed tricycle 5.4km time trial CP div 2 |
| {{bronze medal}} | Michal Stark | Cycling | Mixed individual pursuit LC3 |
| {{bronze medal}} | Pavel Machala | Swimming | Men's 50m freestyle S9 |
| {{bronze medal}} | Martin Kovar | Swimming | Men's 100m freestyle S3 |
| {{bronze medal}} | Jan Povysil | Swimming | Men's 100m freestyle S4 |
| {{bronze medal}} | Jana Hoffmanova | Swimming | Women's 50m backstroke S3 |
| {{bronze medal}} | Vera Stillnerova | Swimming | Women's 50m butterfly S14 |
| {{bronze medal}} | Jana Hoffmanova | Swimming | Women's 100m freestyle S3 |
| {{bronze medal}} | Katerina Coufalova | Swimming | Women's 200m individual medley SM9 |
| {{bronze medal}} | Martin Zvolanek | Swimming | Men's singles 2 |
| {{bronze medal}} | Frantisek Glazar
Michal Stefanu
Lubomir Trcka | Table tennis | Men's team 4 |
| {{bronze medal}} | Jitka Pivarciova | Table tennis | Women's singles 5 |
1. ^{{harvnb|International Paralympic Committee|2000|p=C.1}}