| 词条 | DNA polymerase V |
| 释义 |
| Name = DNA polymerase V, subunit C | image = | width = | caption = | Organism = Escherichia coli (str. K-12 substr. MG1655) | TaxID = 511145 | Symbol = umuC | AltSymbols = | EntrezGene = 946359 | PDB = | RefSeqmRNA = | RefSeqProtein = NP_415702.1 | UniProt = P04152 | ECnumber = 2.7.7.7 | Chromosome = genome | EntrezChromosome = NC_000913.2 | GenLoc_start = 1230218 | GenLoc_end = 1231866 }}{{Infobox nonhuman protein | Name = DNA polymerase V, subunit D | image = | width = | caption = | Organism = Escherichia coli (str. K-12 substr. MG1655) | TaxID = 511145 | Symbol = umuD | AltSymbols = | EntrezGene = 945746 | PDB = | RefSeqmRNA = | RefSeqProtein = NP_415701.1 | UniProt = P0AG11 | ECnumber = 3.4.21.- | Chromosome = genome | EntrezChromosome = NC_000913.2 | GenLoc_start = 1229926 | GenLoc_end = 1230471 }}DNA Polymerase V (Pol V) is a polymerase enzyme involved in DNA repair mechanisms in prokaryotic bacteria, such as Escherichia coli. It is composed of a UmuD' homodimer and a UmuC monomer, forming the UmuD'2C protein complex.[1] It is part of the Y-family of DNA Polymerases, which are capable of performing DNA translesion synthesis (TLS).[2] Translesion polymerases bypass DNA damage lesions during DNA replication - if a lesion is not repaired or bypassed the replication fork can stall and lead to cell death.[3] However, Y polymerases have low sequence fidelity during replication (prone to add wrong nucleotides). When the UmuC and UmuD' proteins were initially discovered in E. coli, they were thought to be agents that inhibit faithful DNA replication and caused DNA synthesis to have high mutation rates after exposure to UV-light.[2] The polymerase function of Pol V was not discovered until the late 1990s when UmuC was successfully extracted, consequent experiments unequivocally proved UmuD'2C is a polymerase. This finding lead to the detection of many Pol V orthologs and the discovery of the Y-family of polymerases.[4] FunctionPol V functions as a TLS(translesion DNA synthesis) polymerase in E. coli as part of the SOS response to DNA damage.[4] When DNA is damaged regular DNA synthesis polymerases are unable to add dNTPs onto the newly synthesized strand. DNA Polymerase III (Pol III) is the regular DNA polymerase in E. coli. As Pol III stalls unable to add nucleotides to the nascent DNA strand, the cell becomes at risk of having the replication fork collapse and apoptosis to take place.[5] Pol V TLS function depends on association with other elements of the SOS response, most importantly Pol V translesion activity is tightly dependent on the formation of RecA nucleoprotein filaments.[8] Pol V can use TLS on lesions that block replication or miscoding lesions, which modify bases and lead to wrong base pairing. However, it is unable to translate through 5' → 3' backbone nick errors.[6] Pol V also lacks exonuclease activity, thus rendering unable to proofread synthesis causing it to be error prone.[7] SOS ResponseSOS response in E. coli attempts to alleviate the effect of a damaging stress in the cell. The role of Pol V in SOS response triggered by UV-radiation is described as follows:
RegulationPol V is only expressed in the cell during the SOS response. It is very tightly regulated at different levels of protein expression and under different mechanisms to avoid its activity unless absolutely necessary for survival of the cell.[5] Pol V's strict regulation stems from its poor replication fidelity, Pol V is highly mutagenic and it is used as a last resort in DNA repair mechanisms. As such, the expression of the UmuD'2C complex takes 45–50 minutes after UV radiation exposure.[6] Transcriptional regulationTranscription of the SOS response genes is negatively regulated by the LexA repressor. LexA binds to the promoter of the UmuDC operon and inhibits gene transcription.[1] DNA damage in the cell leads to the formation of RecA*. RecA* interacts with LexA and stimulates its proteolytic activity, which leads to the autocleavage of the repressor freeing the operon for transcription. The UmuDC operon is transcribed and translated into UmuC and UmuD.[8] Post-translational regulationThe formation of the UmuD'2C complex is limited by the formation of UmuD' from UmuD.[7] UmuD is made of a polypeptide with 139 amino acid residues that form a stable tertiary structure, however it needs to be post-translationally modified to be in its active form.[1] UmuD has self-proteolytic activity that is activated by RecA, it removes 24 amino acids at the N-terminus, turning it into UmuD'. UmuD' can form a homodimer and associate with UmuC toform the active UmuD'2C complex.[8] Functional regulationUmuD'2C complex is inactive unless associated with RecA*. Pol V directly interacts with RecA* at the 3' tip of the nucleoprotein filament; this is the site of the nascent DNA strand where Pol V restarts DNA synthesis.[5] References1. ^1 2 {{cite journal | vauthors = Sutton MD, Walker GC | title = Managing DNA polymerases: coordinating DNA replication, DNA repair, and DNA recombination | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 98 | issue = 15 | pages = 8342–9 | date = Jul 2001 | pmid = 11459973 | doi = 10.1073/pnas.111036998 | pmc=37441}} {{DNA replication}}{{Serine endopeptidases}}{{Kinases}}{{Enzymes}}{{Portal bar|Molecular and Cellular Biology|border=no}}2. ^1 {{cite journal | vauthors = Yang W | title = Damage repair DNA polymerases Y | journal = Current Opinion in Structural Biology | volume = 13 | issue = 1 | pages = 23–30 | date = Feb 2003 | pmid = 12581656 | doi = 10.1016/S0959-440X(02)00003-9 }} 3. ^{{cite book | last1 = Garrett | first1 = Reginald H. | name-list-format = vanc | title = Biochemistry|date=2013|publisher=Nelson Education|location=Toronto|isbn=9780176502652|page=343|edition=1st Canadian}} 4. ^1 {{cite journal | vauthors = Goodman MF, Woodgate R | title = Translesion DNA polymerases | journal = Cold Spring Harbor Perspectives in Biology | volume = 5 | issue = 10 | pages = a010363 | date = Oct 2013 | pmid = 23838442 | doi = 10.1101/cshperspect.a010363 | pmc=3783050}} 5. ^1 2 3 {{cite journal | vauthors = Fuchs RP, Fujii S | title = Translesion DNA synthesis and mutagenesis in prokaryotes | journal = Cold Spring Harbor Perspectives in Biology | volume = 5 | issue = 12 | pages = a012682 | date = Dec 2013 | pmid = 24296168 | doi = 10.1101/cshperspect.a012682 | pmc=3839610}} 6. ^1 {{cite journal | vauthors = Patel M, Jiang Q, Woodgate R, Cox MM, Goodman MF | title = A new model for SOS-induced mutagenesis: how RecA protein activates DNA polymerase V | journal = Critical Reviews in Biochemistry and Molecular Biology | volume = 45 | issue = 3 | pages = 171–84 | date = Jun 2010 | pmid = 20441441 | doi = 10.3109/10409238.2010.480968 | pmc=2874081}} 7. ^1 {{cite journal | vauthors = Yang W | title = An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η | journal = Biochemistry | volume = 53 | issue = 17 | pages = 2793–803 | date = May 2014 | pmid = 24716551 | doi = 10.1021/bi500019s | pmc=4018060}} 8. ^1 2 {{cite journal | vauthors = Jarosz DF, Beuning PJ, Cohen SE, Walker GC | title = Y-family DNA polymerases in Escherichia coli | journal = Trends in Microbiology | volume = 15 | issue = 2 | pages = 70–7 | date = Feb 2007 | pmid = 17207624 | doi = 10.1016/j.tim.2006.12.004 | url = http://www.cell.com/article/S0966842X06002782/abstract }} 3 : DNA replication|EC 2.7.7|EC 3.4.21 |
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