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词条 Double-disk diffusion test
释义

  1. References

  2. Further reading

{{Orphan|date=February 2013}}

A double-disk diffusion test is a kind of disk diffusion test (to test for the effectiveness of an antimicrobial agent a disk infused with it is placed on a cultivated agar dish of bacteria to see if the antimicrobial agent in the disk inhibits further growth of the bacteria.[1])

The double-disc synergy test (DDST) utilizes two of these disks on the cultivated agar solution, either infused with a different antimicrobial solution.[2]

This test was recommended the standard by the Clinical and Laboratory Standards Institute in 2004 for its use against MRSA.[3] To test for clindamycin resistance certain strains of Staphylococcus aureus bacteria with natural resistance to erythromycin are chosen for cultivation on the gel. The two antimicrobial disks contain erythromycin and clindamycin and are placed about 20 mm apart. This is called a D-zone test, or D test. If a 'D' shape is formed around the clindamycin disk than the isolate is reported as resistant to clindamycin.[4] This occurs due to erythromycin inducing the bacteria's ERM (B) gene, and thus making it resistant to Clindamycin.[5]

References

1. ^{{cite book|last1=Fong|first1=I. W.|last2=Drlica|first2=Karl|title=Antimicrobial Resistance and Implications for the 21st Century|date=2007|publisher=Springer Science & Business Media|isbn=9780387724188|pages=58–59|language=en}}
2. ^{{cite book|last1=Lorian|first1=Victor|title=Antibiotics in Laboratory Medicine|date=2005|publisher=Lippincott Williams & Wilkins|isbn=9780781749831|page=487|language=en}}
3. ^{{cite book|last1=Weigelt|first1=John A.|title=MRSA|date=2007|publisher=CRC Press|isbn=9781420045505|page=49|language=en}}
4. ^{{cite book|last1=Malone|first1=Michael A.|title=Infectious Disease, an Issue of Primary Care Clinics in Office Practice|date=2013|publisher=Elsevier Health Sciences|isbn=9780323188685|page=642|language=en}}
5. ^{{cite web|title=Double Disk Diffusion Test (D-Test)|url=http://www.parn.org.pk/index_files/D.test.html|website=www.parn.org.pk|accessdate=1 November 2017}}

Further reading

  • {{cite journal | pmid=23484235 | volume=15 | title=Inducible clindamycin resistance in β-hemolytic streptococci and Streptococcus pneumoniae | year=2013 | journal=Isr. Med. Assoc. J. | pages=27–30 |vauthors=Megged O, Assous M, Weinberg G, Schlesinger Y }}
  • Steward C. et al. Testing for Induction of Clindamycin Resistance in Erythromycin-Resistant Isolates of Staphylococcus aureus. Journal of Clinicl Microbiology. April, 2005.

2 : Bacteriology|Staphylococcus

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