| 词条 | Fluo-3 |
| 释义 |
| ImageFile = Fluo-3.svg | ImageSize = 200px | IUPACName = {[2-(2- | OtherNames = |Section1={{Chembox Identifiers | CASNo = 123632-39-3 | CASNo_Ref = {{cascite|correct|}} | PubChem = 104978 | ChemSpiderID = 94730 | ChEMBL = 509919 | SMILES = O=C(O)CN(c5ccc(cc5OCCOc4c(N(CC(=O)O)CC(=O)O)ccc(C=1c3c(OC=2C=1\\C=C(\\Cl)C(=O)C=2)cc(O)c(Cl)c3)c4)C)CC(=O)O | InChI = 1/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50) | InChIKey = OZLGRUXZXMRXGP-UHFFFAOYAI | StdInChI = 1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50) | StdInChIKey = OZLGRUXZXMRXGP-UHFFFAOYSA-N |Section2={{Chembox Properties | C=36 | H=30 | Cl=2 | N=2 | O=13 | Appearance = | Density = | MeltingPt = | BoilingPt = | Solubility = |Section3={{Chembox Hazards | MainHazards = | FlashPt = | AutoignitionPt = }}Fluo-3 is a fluorescence indicator of intracellular calcium (Ca2+). It is used to measure Ca2+ inside living cells in flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluo-3 is an essentially nonfluorescent compound, but upon binding of Ca2+ its fluorescence increases sharply with an emission maximum at 525 nm suitable for conventionally used detectors designed for fluorescein isothiocyanate (FITC) measurements. This large change in fluorescence coupled with a good yield of photons provides very high contrast which allowed the detection of microscopic Ca2+ release events inside cells called "Calcium sparks".[1] Whereas the salts of fluo-3 are unable to penetrate cells, loading can be achieved using its acetoxymethyl (AM) ester derivative. Once inside the cell, unspecific esterases cleave the ester effectively trapping fluo-3.[2] As calcium is a key second messenger within cells, the specific properties of fluo-3 enable researchers to investigate the time-resolved dynamics of intracellular signal transduction in a diverse range of cells.[3][4] References1. ^{{cite journal | last1 = Cheng | first1 = H. | last2 = Lederer | first2 = W.J. | last3 = Cannell | first3 = M.B. | year = 1993 | title = Calcium Sparks - Elementary Events Underlying Excitation-Contraction Coupling in Heart-Muscle | url = | journal = Science | volume = 262 | issue = 5134| pages = 740–744 | pmid = 8235594 | doi=10.1126/science.8235594}} 2. ^Haugland, RP. Handbook of Fluorescent Probes and Research Products. Molecular Probes, 2010 3. ^Gamsjäger, T. Flow Cytometry of Intracellular Calcium in Platelets. Grin, 2012 4. ^Lambert, DG. Calcium Signaling Protocols. Humana Press, 2006 6 : Cell imaging|Fluorone dyes|Acetic acids|Anilines|Phenol ethers|Chloroarenes |
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