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词条 GelGreen
释义

  1. See also

  2. References

{{chembox
| verifiedrevid =
| ImageFile = GelGreen_chemical_structure.png
| ImageSize =
| ImageFile1 =
| ImageFile2 =
| IUPACName = 10,10'-(6,22-dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis(3,6-bis(dimethylamino)acridin-10-ium) iodide
| OtherNames = Dye No. 20,[1] AOAO-13[1]
|Section1={{Chembox Identifiers
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID = 10032003
| PubChem = 11857532
| UNII_Ref =
| UNII =
| ChEMBL_Ref =
| ChEMBL =
| CASNo_Ref =
| CASNo =
| EINECS =
| ChEBI_Ref =
| ChEBI =
| SMILES =O=C(NCCCOCCOCCOCCCNC(CCCCC[N+]1=C2C(C=CC(N(C)C)=C2)=CC3=C1C=C(N(C)C)C=C3)=O)CCCCC[N+]4=C5C(C=CC(N(C)C)=C5)=CC6=C4C=C(N(C)C)C=C6.[I-].[I-]
| InChI = 1/C56H78N8O5.2HI/c1-59(2)47-23-19-43-37-44-20-24-48(60(3)4)40-52(44)63(51(43)39-47)29-13-9-11-17-55(65)57-27-15-31-67-33-35-69-36-34-68-32-16-28-58-56(66)18-12-10-14-30-64-53-41-49(61(5)6)25-21-45(53)38-46-22-26-50(62(7)8)42-54(46)64;;/h19-26,37-42H,9-18,27-36H2,1-8H3;2*1H
| InChIKey = TWYVVGMYFLAQMU-UHFFFAOYAG
| StdInChI = 1S/C56H78N8O5.2HI/c1-59(2)47-23-19-43-37-44-20-24-48(60(3)4)40-52(44)63(51(43)39-47)29-13-9-11-17-55(65)57-27-15-31-67-33-35-69-36-34-68-32-16-28-58-56(66)18-12-10-14-30-64-53-41-49(61(5)6)25-21-45(53)38-46-22-26-50(62(7)8)42-54(46)64;;/h19-26,37-42H,9-18,27-36H2,1-8H3;2*1H
| StdInChIKey = TWYVVGMYFLAQMU-UHFFFAOYSA-N
| RTECS =
| KEGG_Ref =
| KEGG =
|Section2={{Chembox Properties
| Formula = C56H80I2N8O5
| MolarMass = 1198.43 g/mol
| Appearance =
| Density =
| MeltingPt =
| BoilingPt =
| Solubility =
|Section3={{Chembox Hazards
| ExternalSDS = 10,000X in water, Biotium Inc.
| MainHazards =
| FlashPt = >
| FlashPtC = 100
| AutoignitionPt =
| NFPA-H = 0
| NFPA-F = 0
| NFPA-R = 0
| NFPA-S =
| RPhrases = {{R25}} {{R36/37/38}}
| SPhrases = {{S22}} {{S24/25}} {{S26}} {{S36/37/39}} {{S45}} {{S53}}
}}GelGreen is an intercalating nucleic acid stain used in molecular biology for agarose gel electrophoresis. GelGreen is structurally closely related to acridinium orange and consists of two acridinium orange subunits that are bridged by a linear spacer.[1][2]

Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ultraviolet light, it will fluoresce with a greenish color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelGreen is sold as a solution in DMSO or, more recently, in water.[3]

See also

  • Ethidium bromide
  • GelRed
  • SYBR Green I
  • Agarose gel electrophoresis and gel electrophoresis of nucleic acids
  • Acridine orange

References

1. ^{{cite patent| country = US| number = 2010323453| status = application| title = Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology| pubdate =| gdate =| fdate =| pridate =| inventor =| invent1 = Mao, Fei| invent2 = Leung, Wai-Yee| assign1 =| assign2 =| class = }}
2. ^{{Citation| url = https://biotium.com/wp-content/uploads/2015/12/GelRed-GelGreen-PAGE-Gel-Stains-flyer.pdf| title = GelRed & GelGreen| date = August 21, 2012| publisher = Biotium Inc.| accessdate = December 4, 2012}}
3. ^{{Citation| url = https://biotium.com/technology/gelred-gelgreen-nucleic-acid-gel-stains/| title = GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr| publisher = Biotium Inc.| accessdate = December 4, 2012}}

7 : Aromatic amines|Iodides|DNA-binding substances|Carboxamides|Quaternary ammonium compounds|Acridine dyes|Staining dyes

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