| 词条 | Glycoside hydrolase family 48 |
| 释义 |
| Symbol = Glyco_hydro_48 | Name = Glyco_hydro_48 | image = PDB 1g9j EBI.jpg | width = | caption = x-tal structure of the mutant e44q of the cellulase cel48f in complex with a thiooligosaccharide | Pfam = PF02011 | Pfam_clan = CL0059 | InterPro = IPR000556 | SMART = | PROSITE = | MEROPS = | SCOP = 1fce | TCDB = | OPM family = | OPM protein = | CAZy = GH48 | CDD = }} In molecular biology, glycoside hydrolase family 48 is a family of glycoside hydrolases. Glycoside hydrolases {{EC number|3.2.1.}} are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]Glycoside hydrolase family 48 CAZY GH_48 comprises enzymes with several known activities; endoglucanase ({{EC number|3.2.1.4}}); cellobiohydrolase ({{EC number|3.2.1.91}}). An example of an enzyme containing a domain belonging to this family is one of the cellulases (celA) from the genome of the thermophilic anaerobic bacterium Caldocellum saccharolyticum. The celA gene product is a polypeptide of 1751 amino acids; this has a multidomain structure comprising two catalytic domains and two cellulose-binding domains, linked by Pro-Thr-rich regions. The N-terminal domain encodes an endoglucanase activity on carboxymethylcellulose, consistent with its similarity to several endo-1, 4-beta-D-glucanase sequences, and is a member of the glycoside hydrolase family 9. The C-terminal domain belongs to this family shows similarity to a cellulase from Clostridium thermocellum (CelS), which acts synergistically with a second component to hydrolyse crystalline cellulose.[8] References1. ^{{cite journal | vauthors = Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G | title = Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 92 | issue = 15 | pages = 7090–4 | date = July 1995 | pmid = 7624375 | pmc = 41477 | doi = 10.1073/pnas.92.15.7090 }} {{InterPro content|IPR000556}}{{Sugar hydrolases}}{{Enzymes}}{{Portal bar|Molecular and Cellular Biology|border=no}}2. ^{{cite journal | vauthors = Davies G, Henrissat B | title = Structures and mechanisms of glycosyl hydrolases | journal = Structure | volume = 3 | issue = 9 | pages = 853–9 | date = September 1995 | pmid = 8535779 | doi = 10.1016/S0969-2126(01)00220-9 }} 3. ^{{cite journal | vauthors = Henrissat B, Bairoch A | title = Updating the sequence-based classification of glycosyl hydrolases | journal = The Biochemical Journal | volume = 316 ( Pt 2) | issue = Pt 2 | pages = 695–6 | date = June 1996 | pmid = 8687420 | pmc = 1217404 }} 4. ^{{Cite web|url=http://www.cazy.org/|title=Home|last=|first=|date=|website=CAZy.org|language=en|archive-url=|archive-date=|dead-url=|access-date=2018-03-06}} 5. ^{{cite journal | vauthors = Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B | title = The carbohydrate-active enzymes database (CAZy) in 2013 | journal = Nucleic Acids Research | volume = 42 | issue = Database issue | pages = D490-5 | date = January 2014 | pmid = 24270786 | doi = 10.1093/nar/gkt1178 | url = https://academic.oup.com/nar/article/42/D1/D490/1057423 }} 6. ^{{Cite web|url=http://www.cazypedia.org/index.php/Glycoside_Hydrolase_Family_48|title=Glycoside Hydrolase Family 48|last=|first=|date=|website=CAZypedia.org|language=en|archive-url=|archive-date=|dead-url=|access-date=2018-03-06}} 7. ^{{cite journal | vauthors = | title = Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes | journal = Glycobiology | volume = 28 | issue = 1 | pages = 3–8 | date = December 2018 | pmid = 29040563 | doi = 10.1093/glycob/cwx089 }} 8. ^{{cite journal | vauthors = Te'o VS, Saul DJ, Bergquist PL | title = celA, another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum | journal = Applied Microbiology and Biotechnology | volume = 43 | issue = 2 | pages = 291–6 | year = 1995 | pmid = 7612247 | doi = 10.1007/BF00172827 }} 3 : EC 3.2.1|GH family|Protein families |
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