词条 | Indirect immunoperoxidase assay |
释义 |
MethodologySusceptible cells are inoculated with serial logarithmic dilutions of samples in a 96-well plate. After viral growth, viral detection by IPA yields the infectious virus titer, expressed as tissue culture infectious dose (TCID50). This represents the dilution of a virus-containing sample at which half of a series of laboratory wells contain replicating viruses. This technique is a reliable method for the titration of human coronaviruses (HCoV) in biological samples (cells, tissues, or fluids). It is also reliable in the detection of antibodies to human cytomegalovirus.[1][2] See also
References1. ^1 {{cite book | doi = 10.1007/978-1-59745-181-9_8| chapter = Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay| title = SARS- and Other Coronaviruses| volume = 454| pages = 93| series = Methods in Molecular Biology| year = 2008| last1 = Lambert| first1 = Francine| last2 = Jacomy| first2 = Hélène| last3 = Marceau| first3 = Gabriel| last4 = Talbot| first4 = Pierre J| isbn = 978-1-58829-867-6}} 2. ^G Gerna, C J McCloud and R W Chambers: Immunoperoxidase technique for detection of antibodies to Human cytomegalovirus. J. Clin. Microbiol. 1976, 3(3):364. External links
4 : Virology|Biochemistry methods|Immunologic tests|Laboratory techniques |
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