“Rubredoxin A”的意思、由来-开放百科全书

As of March 2015 there was no crystal structure of RubA although a structure of the homologous protein from a cryptomonad was determined using NMR.^[2] Investigation of the gene however indicates that it differs from other known rubredoxins in being bound to the thylakoid membrane via a C-terminal transmembrane helix.^[1]^[3]^[4]

Function

An investigation of Guillardia theta noted that RubA had a similar distribution to Photosystem II (PSII) and immunological experiments indicated the presence of RubA in PSII complexes isolated from Spinacia oleracea.^[3] In Synechocystis sp. PCC 6803 it has been demonstrated that the insertion of an antibiotic cassette into the rubA gene results in a marked decrease in the amount of PSII present,^[1] while the same mutation within Chlamydomonas reinhardtii and in Arabidopsis thaliana results in a total absence of PSII.^[1]

Conversely, another study performed in Synechococcus sp. PCC 7002 indicated that mutation of the rubA gene interfered with iron-sulphur cluster assembly in Photosystem I (PSI), rather than affecting PSII.^[4]^[5]

References

1. ^¹²³{{cite journal | author = Calderon, R. H., García-Cerdán, J. G., Malnoë, A., Cook, R., Russell, J. J., Gaw, C., Dent, R. M., de Vitry, C. and Niyogi, K. K. | title = A Conserved Rubredoxin Is Necessary for Photosystem II Accumulation in Diverse Oxygenic Photoautotrophs | journal = The Journal of Biological Chemistry | volume = 288 | pages = 26688–26696 | date = July 2013 | doi = 10.1074/jbc.M113.487629 | pmid=23900844 | pmc=3772215}}
2. ^{{cite journal | author = Schweimer, K., Hoffmann, S., Wastl, J., Maier, U.G., Rösch, P., and Sticht H. | title = Solution structure of a zinc substituted eukaryotic rubredoxin from the cryptomonad alga "Guillardia theta"| journal = Protein Science | volume = 9 | pages = 1474–1486 | doi = 10.1110/ps.9.8.1474 | pmid=10975569 | pmc=2144721}}
3. ^¹{{cite journal | author = Wastl, J., Duin, E. C., Iuzzolino, L., Dörner, W., Link. T., Dau, H., Lingelbach, K. and Maier U. G. | title = Eukaryotically Encoded and Chloroplast-located Rubredoxin Is Associated with Photosystem II | journal = The Journal of Biological Chemistry | volume = 275 | pages = 30058–30068 | doi = 10.1074/jbc.M004629200 | pmid=10878021}}
4. ^¹{{cite journal | author = Shen, G., Zhao, J., Reimer, S. K., Antonkine, M. L., Cai, Q., Weiland, S. M., Golbeck, J. H. and Bryant, D. A. | title = Assembly of Photosystem II: I. Inactivation of The rubA Gene Encoding A Membrane-Associated Rubredoxin In The Cyanobacterium Synechococcus sp. PCC 7002 Causes A Loss of Photosystem I Activity | journal = The Journal of Biological Chemistry | volume = 277 | pages = 20343–20354 | doi = 10.1074/jbc.M201103200 | pmid=11914373}}
5. ^{{cite journal | author = Shen, G., Antonkine, M. L., van der Est, A., Vassiliev, I. R., Brettel, K., Bittl, R., Zech, S. G., Zhao, J., Stehlik, D., Bryant, D. A. and Golbeck, J. H. | title = Assembly of Photosystem I: II. Rubredoxin Is Required for the Assembly of Fx in Synechococcus sp. PCC 7002 As Shown by Optical and EPR Spectroscopy | journal = The Journal of Biological Chemistry | volume = 277 | pages = 20355–20366 | doi = 10.1074/jbc.M201104200}}

Structure

Function

References