Thursday 23 September 1909 – 10 laps (158 ⅛ miles) St. John's Short Course.
| Rank | No | Rider | Team | Speed | Time |
|---|
| 1 | 18 | {{flagicon|United Kingdom}} Harry Collier | 6 hp Matchless | 49.01 mph | 3:13.37.8 |
| 2 | 7 | {{flagicon|United Kingdom}} W.Lee Evans | 5 hp Indian | 48.07 mph | 3:17.35.2 |
| 3 | 11 | {{flagicon|United Kingdom}} Oliver Godfrey | 3½ hp Rex | 44.89 mph | 3:31.31.6 |
| 4 | 5 | {{flagicon|United Kingdom}} C.B.Franklin | 3½ hp Triumph | 43.06 mph | 3:40.31.2 |
| 5 | 45 | {{flagicon|United Kingdom}} W.F.Newsome | 3½ hp Triumph | 42.92 mph | 3:41.10.0 |
| 6 | 10 | {{flagicon|United Kingdom}} F.A.Applebee | 5½ hp Rex | 42.69 mph | 3:42.24.2 |
| 7 | 34 | {{flagicon|United Kingdom}} B.R.Jones | 3¾ hp Premier | 41.87 mph | 3:46.54.2 |
| 8 | 38 | {{flagicon|United Kingdom}} A.J.Moorhouse | 5 hp Rex | 41.88 mph | 3:46.54.6 |
| 9 | 19 | {{flagicon|United Kingdom}} H.V.Colver | 3½ hp Matchless | 40.23 mph | 3:55.08.6 |
| 10 | 17 | {{flagicon|United Kingdom}} J.D.A.Munroe | 5 hp BAT | 41.95 mph | 3:46.15.2 |
| 11 | 46 | {{flagicon|United Kingdom}} W.Creyton | 3½ hp Triumph | 40.36 mph | 3:55.10.0 |
| 12 | 54 | {{flagicon|France}} J.Giuoppone | 3½ hp Peugeot | 39.95 mph | 3:57.49.0 |
| 13 | 55 | {{flagicon|United Kingdom}} J.Lang | 5 hp Tee-Bee | 39.44 mph | 4:00.53.0 |
| 14 | 17 | {{flagicon|United Kingdom}} Jas Stewart | 3½ hp Triumph | 37.88 mph | 4:10.45.5 |
| 15 | 49 | {{flagicon|United Kingdom}} A.D.Blake | 3 hp Triumph | 38.84 mph | 4:04.02.8 |
| 16 | 39 | {{flagicon|United Kingdom}} Rem Fowler | 5 hp Rex | 37.14 mph | 4:15.47.6 |
| 17 | 40 | {{flagicon|United Kingdom}} T.Greer | 5 hp Rex | 34.93 mph | 4:25.59.6 |
| 18 | 25 | {{flagicon|United Kingdom}} Bert Sproston | 5 hp Rex | 32.13 mph | 4:55.25.2 |
1. ^Isle of Man Weekly Times pp4 dated 30 September 1909