| 词条 | Tityustoxin peptide 2 | ||||||||||||
| 释义 |
|name = Infobox TsPep2 |bodystyle = |title = Tityustoxin peptide 2 |titlestyle = |headerstyle = background:#ccf; |labelstyle = background:#ddf; |header1 = SCOP classification[2] |label2 = Class |data2 = Small proteins |label3 = Superfamily |data3 = Scorpion toxin-like |label4 = Family |data4 = Short-chain scorpion toxin[3] |label5 = Species |data5 = Tityus serrulatus |label6 = UniProt |data6 = P0C175[4] }} SourcesTityus serrulatus, also known as the Brazilian yellow scorpion, is from the genus Tityus belonging to the family Buthidae. TsPep2 is identified from the venom of Tityus serrulatus by using a cDNA primer sequence based on the C-terminal amino acid sequence of KTx2 from Androctonus australis.[1][5] ChemistryThe original encoded sequence of TsPep2 consists of 68 amino acids processed in a mature peptide of 29 amino acids with a final molecular weight of 2993.59 Da.[1][6] TsPep2 differs in the mature sequence from TsPep3 only in one amino acid and TsPep1 shows 58,6% of sequence homology with Tspep2 and TsPep3.[1]
Eight cysteines establish four disulfide bridges and a C-terminal tyrosine amide is present in the 55th position. Furthermore, TsPep2 sequence alignment shows that a part of the amino acid consensus sequence (CXXXKCCXC) involved in the pore blocking mechanism is present as in other known short scorpion toxins.[1][6][7] TargetIt seems likely that TsPep2 has inhibitory actions on potassium channels, based on its sequence similarities in the C-terminal beta sheet with the potassium channel inhibitory alpha family (α-KTX) and on its similarities in the patterns of disulfide bridges with other short scorpion venom toxins.[1][6][8] ToxicityThe biological function of TsPep2 is not clear yet, except from a small displacement on the 125I-KTX binding site on rat brain synaptosomes.[8] However, it has been shown that this peptide is not toxic to mice.[1][4] The LD50 of TsPep2 is currently unknown. References1. ^1 2 3 4 5 6 {{cite journal|last1=Pimenta|first1=Adriano M.C.|last2=Legros|first2=Christian|last3=Almeida|first3=Flavia de Marco|last4=Mansuelle|first4=Pascal|last5=Bougis|first5=Pierre E.|last6=Martin-Eauclaire|first6=Marie F.|title=Novel structural class of four disulfide-bridged peptides from Tityus serrulatus venom|journal=Biochemical and Biophysical Research Communications|volume=301|issue=4|year=2003|pages=1086–1092|doi=10.1016/S0006-291X(03)00082-2}} 2. ^{{cite web|url=http://scop.berkeley.edu/sunid=242892|title=SCOPe 2.05: Domain d2li7a_: 2li7 A:|publisher=scop.berkeley.edu|accessdate=2016-02-10}} 3. ^{{cite web|url=http://supfam.cs.bris.ac.uk/SUPERFAMILY/cgi-bin/scop.cgi?sunid=57116|title= Superfamily - Tityustoxin peptide 2 |publisher=supfam.cs.bris.ac.uk|accessdate=2016-02-09}} 4. ^1 2 {{cite web|url=https://www.uniprot.org/uniprot/P0C175|title=Tityustoxin peptide 2 - Tityus serrulatus (Brazilian yellow scorpion)|publisher=uniprot.org|accessdate=2016-09-25}} 5. ^{{cite web|url=https://books.google.nl/books?id=_ZizxAyUMIcC&pg=PP12&lpg=PP12&dq=Structure-function+relationship+of+K2Bion+channel+toxins:+from+cloning+to+functional+characterization.&source=bl&ots=DUlUG95BBD&sig=TWsV1HYQsaSaN8RBkiSaODDhazg&hl=en&sa=X&ved=0ahUKEwi6_LDHltDPAhUDtBoKHXqeD9sQ6AEIITAA#v=onepage&q=Structure-function%20relationship%20of%20K%2Bion%20channel%20toxins%3A%20from%20cloning%20to%20functional%20characterization.&f=false|title=Structure function relationship of K+ion channel toxins:from cloning to functional characterization |publisher=Isabelle Huys |pages=22–27 |location=Leuve, Belgium |year=2004}} 6. ^1 2 {{cite web|url=https://www.ebi.ac.uk/interpro/entry/IPR008911|title=Family relationships - Tityustoxin peptide 2|publisher=ebi.ac.uk|accessdate=2016-02-10}} 7. ^{{cite journal|last1=Almeida|first1=almeida.|last2=Diego D.|first2=Scortecci|last3=Katia C.|first3=Kobashi|last4=Leonardo S.|first4=Agnes-Lima|last5=Lucymara F|first5=Fernandes Pedrosa.|year=2012|title=Profiling the resting venom gland of the scorpion Tityus stigmurus through a transcriptomic survey|journal= BMC Genomics |volume=13|page=362|doi=10.1186/1471-2164-13-362|pmid=22853446|pmc=3444934}} 8. ^1 {{cite journal|last1=Eauclaire|first1=Martin|last2= France |first2=Marie|last3=Pimenta|first3=Adriano M. C.|last4=Bougis|first4=Pierre E|last5=de Lima|first5=Maria-Elenal | year=2016|title=Potassium channel blockers from the venom of the Brazilian scorpion Tityus serrulatus|journal= Toxicon |volume=119| pages=253–265|doi=10.1016/j.toxicon.2016.06.016 |pmid=27349167}} 1 : Ion channel toxins |
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