| 词条 | Cell fractionation |
| 释义 |
HomogenizationTissue is typically homogenized in a buffer solution that is isotonic to stop osmotic damage. Mechanisms for homogenization include grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultra-sound. The samples are then kept cold to prevent enzymatic damage. It is the formation of homogenous mass of cells (cell homogenate or cell suspension). It involves grinding of cells in a suitable medium in the presence of certain enzymes with correct pH, ionic composition, and temperature. For example, pectinase which digests middle lamella among plant cells. FiltrationThis step may not be necessary depending on the source of the cells. Animal tissue however is likely to yield connective tissue which must be removed. Commonly, filtration is achieved either by pouring through gauze or with a suction filter and the relevant grade ceramic filter. PurificationPurification is achieved by differential centrifugation – the sequential increase in gravitational force results in the sequential separation of organelles according to their density. See also
Media for cell separation by density:
References1. ^{{cite web|last1=Alberts|first1=B|last2=Johnson|first2=A|title=Fractionation of Cells|url=https://www.ncbi.nlm.nih.gov/books/NBK26936/|publisher=Molecular Biology of the Cell. 4th edition.}} 2. ^{{Cite book|title=Fundamental Laboratory Approaches for Biochemistry and Biotechnology|last=Ninfa|first=Alexander J.|publisher=John Wiley & Sons, INC.|year=2010|isbn=978-0-470-08766-4|location=United States of America|pages=209}} 2 : Laboratory techniques|Fractionation |
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