Division Two may refer to:

• Football League Two, the current fourth tier of the English football league system
• Football League Second Division, the former second and later third tier of the English football league system

• Cuban 8
• Cuban abstract painters
• Cuban Academy of Science
• Cubana Cargo
• Cubana de Aviacion destinations
• Cubana de Aviacion Flight 1216
• Cubana de Aviacion Flight 310
• Cubana de Aviación Flight 1216
• Cubana de Aviación Flight 310
• Cubana de Aviación S.A.
• Cuban agriculture
• Cuban Amateur Radio Federation
• Cuban America
• Cuban-American Treaty
• Cuba-Namibia relations
• Cuban anarchism
• Cuban anarchists
• Cuban anole
• Cuban Anole (disambiguation)
• Cuban Arco Progresista (Social-Democratic) Party
• Cuban armed forces
• Cuban army
• Cuban Assassin (disambiguation)
• Cuban Assets Control Regulations
• Cuban assistance to the FSLN